| Description |
1 online resource (xiv, 417 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; v. 1834 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1834. 1064-3745
|
| Contents |
Identification and analysis of genes associated with inherited retinal diseases / Mubeen Khan, Zeinab Fadaie, Stéphanie S. Cornelis, Frans P.M. Cremers, and Susanne Roosing -- Conduct and quality control of differential gene expression analysis using high-throughput transcriptome sequencing (RNASeq) / Felix Grassmann -- Testing for known retinal degeneration mutants in mouse strains / Khalid Rashid, Katharina Dannhausen, and Thomas Langmann -- CRISPR/Cas9 gene editing in vitro and in retinal cells in vivo / Daniela Benati, Valeria Marigo, and Alessandra Recchia -- Monitoring surface reactions by combined western blot-ELISA analysis / Yuchen Lin, Sarah Irmscher, and Christine Skerka -- Generation of functional retinal pigment epithelium from human induced pluripotent stem cells / Caroline Brandl -- Advanced analysis of photoreceptor outer segment phagocytosis by RPE cells in culture / Francesca Mazzoni, Yingyu Mao, and Silvia C. Finnemann -- Porcine RPE/choroidal explant cultures / Alexa Klettner and Yoko Miura -- Mouse retinal organoid trisection recipe : efficient generation of 3D retinal tissue from mouse embryonic stem cells / Manuela Völkner, Thomas Kurth, and Mike O. Karl -- Cell death analysis in retinal cultures / Sarah L. Roche, Ana M. Ruiz-Lopez, and Thomas G. Cotter -- Fate mapping in vivo to distinguish bona fide microglia versus recruited monocyte-derived macrophages in retinal disease / Nancy J. Reyes, Rose Mathew, and Daniel R. Saban -- Light damage models of retinal degeneration / Christian Grimm and Charlotte E. Remé -- Induction and readout of oxygen-induced retinopathy / Raffael Liegl, Claudia Priglinger, and Andreas Ohlmann -- Generation and analysis of Xenopus laevis models of retinal degeneration using CRISPR/Cas9 / Joanna M. Feehan, Paloma Stanar, Beatrice M. Tam, Colette Chiu, and Orson L. Moritz -- Gene knockdown in zebrafish (Danio rerio) as a tool to model photoreceptor diseases / Holger Dill and Utz Fischer -- Drosophila melanogaster : a valuable genetic model organism to elucidate the biology of retinitis pigmentosa / Malte Lehmann, Elisabeth Knust, and Sarita Hebbar -- Retinal fundus imaging in mouse models of retinal diseases / Anne F. Alex, Maged Alnawaiseh, Peter Heiduschka, and Nicole Eter -- Phenotyping of mouse models with OCT / G. Alex Ochakovski and M. Dominik Fischer -- Cell-specific markers for the identification of retinal cells and subcellular organelles by immunofluorescence microscopy / Laurie L. Molday, Christiana L. Cheng, and Robert S. Molday -- Immuno-TEM/STEM in retinal research / Sanae Sakami and Krzysztof Palczewski -- Noninvasive two-photon microscopy imaging of mouse retina and retinal pigment epithelium / Grazyna Palczewska, Timothy S. Kern, and Krzysztof Palczewski -- Analysis of the Drosophila compound eye with light and electron microscopy / Monalisa Mishra and Elisabeth Knust -- Cell-based therapy for retinal disease : the new frontier / Marco Zarbin -- In vitro evaluation of AAV vectors for retinal gene therapy / Johanna E. Wagner, Christian Schön, Elvir Becirovic, Martin Biel, and Stylianos Michalakis -- Nanoparticles targeting retinal and choroidal capillaries in vivo / Alexandra Haunberger and Achim Goepferich -- Optimized subretinal injection technique for gene therapy approaches / Regine Mühlfriedel, Stylianos Michalakis, Marina Garcia Garrido, Vithiyanjali Sothilingam, Christian Schön, Martin Biel, and Mathias W. Seeliger |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title pate (SpringerLink, viewed October 16, 2018) |
| Subject |
Retinal degeneration -- Laboratory manuals
|
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Retina.
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Retinal degeneration.
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Retina -- Diseases.
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Retina
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Retinal Degeneration
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Retinal Diseases
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Genetic Therapy -- methods
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Medical research.
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Retina -- Diseases
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Retina
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Retinal degeneration
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| Genre/Form |
Electronic books
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Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Weber, Bernhard H. F., editor
|
|
Langmann, Thomas, editor
|
| ISBN |
9781493986699 |
|
1493986694 |
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