| Description |
1 online resource (xi, 376 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 2351 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; 2351. 1064-3745
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Springer protocols (Series) 1949-2448
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| Contents |
A New Toolbox to Analyze Enhancer-Promoter Functions -- Global Run-on Sequencing (GRO-Seq) -- Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET-Seq) -- Low Quantity single strand CAGE (LQ-ssCAGE) Maps Regulatory Enhancers and Promoters -- Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase-Seq -- Measuring Chromatin Accessibility: ATAC-Seq -- High-resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles -- Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes -- Low Input Targeted Chromatin Capture (low-T2C) -- Proximity Ligation-assisted ChIP-Seq (PLAC-Seq) -- Analysis of Enhancer-promoter Interactions using CAGE and RADICL-Seq technologies -- Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to identify open chromatin interactions -- Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C) -- Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-Translational Modification (PTM) Combinatorics At Distinct Genomic Regions -- Using ChIP-SICAP to Identify Proteins That Co-Localize in Chromatin -- Genome-wide profiling of protein-DNA Interactions with Chromatin Endogenous Cleavage And High-Throughput Sequencing (ChEC-Seq) -- Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators -- Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors -- Simultaneous Tagmentation-based detection of ChIP/ATAC Signal with Bisulfite Sequencing -- Low Input Whole-Genome Bisulfite Sequencing |
| Summary |
This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays. Written in the Methods in Molecular Biology series format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting. Authoritative and cutting-edge, Enhancers and Promoters: Methods and Protocols is a useful guide for future experiments |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed August 18, 2021) |
| Subject |
Gene expression.
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Genetic regulation.
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Gene Expression
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Gene Expression Regulation
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Gene expression
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Genetic regulation
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Borggrefe, Tilman, editor
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Giaimo, Benedetto Daniele, editor
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| ISBN |
9781071615973 |
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1071615971 |
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