| Description |
1 online resource (xv, 472 pages) : illustrations |
| Contents |
Machine generated contents note: Abbreviations / Haleem J. Issaq / Timothy D. Veenstra -- Introduction / Haleem J. Issaq / Timothy D. Veenstra -- Definitions / Timothy D. Veenstra / Haleem J. Issaq -- The Current State of Biomarker Discovery / Timothy D. Veenstra / Haleem J. Issaq -- Study Design and Execution / Timothy D. Veenstra / Haleem J. Issaq -- Errors in Study Design / Haleem J. Issaq / Timothy D. Veenstra -- Errors in Study Execution / Timothy D. Veenstra / Haleem J. Issaq -- Specificity of Proteins as Biomarkers / Haleem J. Issaq / Timothy D. Veenstra -- Statistical Data Analysis / Timothy D. Veenstra / Haleem J. Issaq -- Recommendations / Haleem J. Issaq / Timothy D. Veenstra -- Concluding Remarks and Recommendations / Timothy D. Veenstra / Haleem J. Issaq -- Acknowledgments / Haleem J. Issaq / Timothy D. Veenstra -- References / Timothy D. Veenstra / Haleem J. Issaq -- Introduction / Maria P. Pavlou / Ihor Batruch / Eleftherios P. Diamandis / Andrei P. Drabovich -- Protein Biomarker Discovery and Development Pipeline / Eleftherios P. Diamandis / Ihor Batruch / Maria P. Pavlou / Andrei P. Drabovich -- Proteomic Samples / Andrei P. Drabovich / Maria P. Pavlou / Ihor Batruch / Eleftherios P. Diamandis -- Protein Identification by Mass Spectrometry / Andrei P. Drabovich / Maria P. Pavlou / Ihor Batruch / Eleftherios P. Diamandis -- Post-Translational Modifications as Disease Biomarkers / Andrei P. Drabovich / Eleftherios P. Diamandis / Ihor Batruch / Maria P. Pavlou -- Protein Quantification by Mass Spectrometry / Andrei P. Drabovich / Eleftherios P. Diamandis / Ihor Batruch / Maria P. Pavlou -- Biomarker Verification / Andrei P. Drabovich / Eleftherios P. Diamandis / Ihor Batruch / Maria P. Pavlou -- Biomarker Validation / Andrei P. Drabovich / Eleftherios P. Diamandis / Ihor Batruch / Maria P. Pavlou -- Limitations of Mass Spectrometry for Protein Biomarker Discovery / Andrei P. Drabovich / Eleftherios P. Diamandis / Ihor Batruch / Maria P. Pavlou -- Conclusions and Future Outlook: Integrated Biomarker Discovery Platform / Andrei P. Drabovich / Eleftherios P. Diamandis / Ihor Batruch / Maria P. Pavlou -- References / Andrei P. Drabovich / Eleftherios P. Diamandis / Ihor Batruch / Maria P. Pavlou -- Introduction / Xiaoying Ye / Darue A. Prieto / Josip Blonder / Bih-Rong Wei / King C. Chan -- Types of Tissues Available for MS-Based Proteomics / Xiaoying Ye / Darue A. Prieto / Josip Blonder / Bih-Rong Wei / King C. Chan -- Tissue Disruption/Homogenization / Xiaoying Ye / Darue A. Prieto / Josip Blonder / Bih-Rong Wei / King C. Chan -- Extraction/Solubilization Buffers / Xiaoying Ye / Darue A. Prieto / Josip Blonder / Bih-Rong Wei / King C. Chan -- Concluding Remarks / Xiaoying Ye / Darue A. Prieto / Josip Blonder / Bih-Rong Wei / King C. Chan -- Acknowledgments / Josip Blonder / Xiaoying Ye / Bih-Rong Wei / King C. Chan / Darue A. Prieto -- References / Xiaoying Ye / Darue A. Prieto / Josip Blonder / Bih-Rong Wei / King C. Chan -- Abbreviations / Dajana Vuckovic -- Introduction / Dajana Vuckovic -- An Ideal Sample Preparation Method for Global Metabolomics? / Dajana Vuckovic -- Sample Preparation Methods for Biofluids / Dajana Vuckovic -- Tissue Metabolomics / Dajana Vuckovic -- New Trends in Sample Preparation for Global Metabolomics / Dajana Vuckovic -- Conclusions and Future Perspective / Dajana Vuckovic -- Acknowledgments / Dajana Vuckovic -- References / Dajana Vuckovic -- Introduction / Melissa Tuck / Dean E. Brenner / D. Kim Turgeon -- Importance of Preanalytical Variables / Melissa Tuck / Dean E. Brenner / D. Kim Turgeon -- Standard Operating Procedures (SOPs) / Melissa Tuck / Dean E. Brenner / D. Kim Turgeon -- Sample Selection Considerations / Melissa Tuck / Dean E. Brenner / D. Kim Turgeon -- Human Blood and Its Components / Melissa Tuck / Dean E. Brenner / D. Kim Turgeon -- Other Biosamples / Melissa Tuck / Dean E. Brenner / D. Kim Turgeon -- Blood-Borne Pathogens, Universal Precautions, and Safety / Melissa Tuck / Dean E. Brenner / D. Kim Turgeon -- Human Subjects Research Protections / Melissa Tuck / Dean E. Brenner / D. Kim Turgeon -- Conclusions / Melissa Tuck / Dean E. Brenner / D. Kim Turgeon -- References / Melissa Tuck / Dean E. Brenner / D. Kim Turgeon -- Introduction: Why NMR? / Que N. Van -- NMR Hardware Advancement / Que N. Van -- Sample Preparation for NMR Analysis / Que N. Van -- One-Dimensional NMR Methods: 1H, 13C, 31P / Que N. Van -- 2D Methods / Que N. Van -- Targeted Metabolic Profiling / Que N. Van -- High-Resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy / Que N. Van -- Magnetic Resonance Spectroscopy (MRS) / Que N. Van -- NMR Data Processing and Preparation for Statistical Analysis / Que N. Van -- NMR Metabolite Identification / Que N. Van -- Future Directions and Conclusion / Que N. Van -- References / Que N. Van -- Introduction / David R. Goodlett / Young Ah Goo -- PAcIFIC and Quantification / David R. Goodlett / Young Ah Goo -- Proteome Profiling with PAcIFIC / David R. Goodlett / Young Ah Goo -- Perspectives / Young Ah Goo / David R. Goodlett -- References / David R. Goodlett / Young Ah Goo -- Introduction / Eric Chun Yong Chan / Lian Yee Yip -- GC/MS in Metabonomics / Eric Chun Yong Chan / Lian Yee Yip -- Strategies to Address Large-Scale Metabonomic Investigations / Eric Chun Yong Chan / Lian Yee Yip -- Conclusion and Future Outlook / Eric Chun Yong Chan / Lian Yee Yip -- References / Eric Chun Yong Chan / Lian Yee Yip -- Introduction / Georgios A. Theodoridis / Ian D. Wilson / Robert Plumb / Helen G. Gika -- Chromatographic Methods for Metabolite Profiling / Georgios A. Theodoridis / Ian D. Wilson / Robert Plumb / Helen G. Gika -- Detection / Georgios A. Theodoridis / Ian D. Wilson / Robert Plumb / Helen G. Gika -- Quality Control, Data Analysis, and Biomarker Detection / Georgios A. Theodoridis / Ian D. Wilson / Robert Plumb / Helen G. Gika -- Metabolite Identification and Biomarker Validation / Georgios A. Theodoridis / Ian D. Wilson / Robert Plumb / Helen G. Gika -- Conclusions / Georgios A. Theodoridis / Ian D. Wilson / Robert Plumb / Helen G. Gika -- References / Georgios A. Theodoridis / Ian D. Wilson / Robert Plumb / Helen G. Gika -- Analysis of Metabolite Profiles Using Capillary Electrophoresis-Mass Spectrometry / Cheng S. Lee / Chenchen Wang -- Analysis of Protein Expression Levels Using Capillary Electrophoresis-Mass Spectrometry / Cheng S. Lee / Chenchen Wang -- Conclusion / Cheng S. Lee / Chenchen Wang -- Acknowledgments / Cheng S. Lee / Chenchen Wang -- References / Cheng S. Lee / Chenchen Wang -- Introduction / Egisto Boschetti / Pier Giorgio Righetti / Giovanni Candiano -- The Evolution of 2-DE Toward Proteomics Applications / Egisto Boschetti / Pier Giorgio Righetti / Giovanni Candiano -- Low-Abundance Proteins Are Not Resolved by 2-DE Alone / Egisto Boschetti / Pier Giorgio Righetti / Giovanni Candiano -- Enhancing Low-Abundance Proteins / Pier Giorgio Righetti / Giovanni Candiano / Egisto Boschetti -- The Discovery of Protein Markers with 2-DE and Its Association with Low-Abundance Protein Enrichment / Pier Giorgio Righetti / Egisto Boschetti / Giovanni Candiano -- Conclusions / Giovanni Candiano / Pier Giorgio Righetti / Egisto Boschetti -- Dedication / Egisto Boschetti / Giovanni Candiano / Pier Giorgio Righetti -- Acknowledgments / Pier Giorgio Righetti / Egisto Boschetti / Giovanni Candiano -- References / Pier Giorgio Righetti / Giovanni Candiano / Egisto Boschetti -- Introduction / Timothy D. Veenstra / Haleem J. Issaq -- Gel Electrophoresis: Historical Perspective / Timothy D. Veenstra / Haleem J. Issaq -- Two-Dimensional Differential In-Gel Electrophoresis / Haleem J. Issaq / Timothy D. Veenstra -- Strengths and Weaknesses of 2D-PAGE and 2D-DIGE / Haleem J. Issaq / Timothy D. Veenstra -- Application of 2D-DIGE to Biomarker Discovery / Haleem J. Issaq / Timothy D. Veenstra -- Conclusions / Timothy D. Veenstra / Haleem J. Issaq -- Acknowledgments / Timothy D |
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Veenstra / Haleem J. Issaq -- References / Haleem J. Issaq / Timothy D. Veenstra -- Introduction / Fred E. Regnier / Wonryeon Cho -- Affinity Targeting Methods / Wonryeon Cho / Fred E. Regnier -- Single Gene Expression Product Selection / Fred E. Regnier / Wonryeon Cho -- Post-Translational Modifications / Fred E. Regnier / Wonryeon Cho -- Abundant Protein Removal / Fred E. Regnier / Wonryeon Cho -- Conclusions / Wonryeon Cho / Fred E. Regnier -- References / Wonryeon Cho / Fred E. Regnier -- Introduction / Joe R. Cannon / Catherine Fenselau -- Aspartate-Selective Acid Proteolysis / Joe R. Cannon / Catherine Fenselau -- Microwave-Supported Asp-Selective Acid Proteolysis / Joe R. Cannon / Catherine Fenselau -- Methods Development with Microwave-Supported Acid Hydrolysis / Joe R. Cannon / Catherine Fenselau -- Applications of Microwave-Supported Acid Hydrolysis / Joe R. Cannon / Catherine Fenselau -- Advantages and Disadvantages of Microwave-Supported Asp-Selective Acid Proteolysis / Joe R. Cannon / Catherine Fenselau -- References / Joe R. Cannon / Catherine Fenselau -- Introduction / Haleem J. Issaq / Timothy D. Veenstra -- Depletion / Haleem J. Issaq / Timothy D. Veenstra -- Fractionation Procedures for Proteins and Metabolites / Haleem J. Issaq / Timothy D. Veenstra -- Affinity Chromatography / Timothy D. Veenstra / Haleem J. Issaq -- Isoelectric Focusing / Timothy D. Veenstra / Haleem J. Issaq -- Size Exclusion Chromatography / Timothy D. Veenstra / Haleem J. Issaq -- Conclusions / Timothy D. Veenstra / Haleem J. Issaq -- References / Timothy D. Veenstra / Haleem J. Issaq -- Protein Identification / Timothy D. Veenstra / Haleem J. Issaq / Stephen D. Fox / Que N. Van -- Metabolite Identification in Global Metabolomics / Timothy D. Veenstra / Haleem J. Issaq / Stephen D. Fox / Que N. Van -- References / Timothy D. Veenstra / Haleem J. Issaq / Stephen D. Fox / Que N. Van -- Introduction / Xudong Yao / Mary Joan Castillo / Adam J. McShane |
| Summary |
Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes. Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research. Describes the use of biomarkers to reduce clinical errors in research. Includes techniques from a range of biomarker discoveries. Covers all steps involved in biomarker discovery, from study design to study execution |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Biochemical markers.
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Proteomics.
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Proteomics -- methods
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Biomarkers
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Metabolomics -- methods
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Proteomics
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MEDICAL -- Allied Health Services -- Medical Technology.
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MEDICAL -- Biotechnology.
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MEDICAL -- Family & General Practice.
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MEDICAL -- Lasers in Medicine.
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TECHNOLOGY & ENGINEERING -- Biomedical.
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Biochemical markers
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Proteomics
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| Form |
Electronic book
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| Author |
Issaq, Haleem J.
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Veenstra, Timothy Daniel, 1966-
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| LC no. |
2013444129 |
| ISBN |
9780123947956 |
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0123947952 |
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1299619185 |
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9781299619180 |
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