| Description |
1 online resource (xi, 338 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; volume 1623 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1623. 1064-3745
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| Contents |
Analysis of the germinal center reaction in tissue sections / David Dominguez-Sola and Giorgio Cattoretti -- Detecting gene expression in lymphoid microenvironments by laser microdissection and quantitative RT-PCR / Yang Zhang, Laura Garcia-Ibanez, Geoffrey Brown, and Kai-Michael Toellner -- Tracking plasma cell differentiation in living mice with two-photon microscopy / Carolin Ulbricht, Randall L. Lindquist, Laura Tech, and Anja E. Hauser -- Microanatomical labeling of germinal center structures for flow cytometry using photoactivation / Johanne T. Jacobsen and Gabriel D. Victora -- Fate mapping and transcript profiling of germinal center cells by two-photon photoconversion / Imogen Moran and Tri Giang Phan -- Intravital microscopy of T-B cell interactions in germinal centers / Changming Shih and Hai Qi -- Identifying follicular regulatory T cells by confocal microscopy / Ine Vanderleyden and Michelle A. Linterman -- Cytokine expression by T follicular helper cells / Christoph Jandl, Claudia Loetsch, and Cecile King -- Follicular dendritic cell isolation and loading of immune complexes / Balthasar A. Heesters, Cees E. Van der Poel, and Michael C. Carroll -- Isolation and characterization of mouse and human follicular dendritic cells / Mohey Eldin M. El Shikh, Riham El Sayed, and Costantino Pitzalis -- In vitro-induced germinal center B cell culture system / Kei Haniuda, Takuya Nojima, and Daisuke Kitamura -- CRISPR/Cas9-mediated in vitro mutagenesis in GC-like B cells / Van Trung Chu, Robin Graf, and Klaus Rajewsky -- Germinal center formation with retrovirally transduced B cells for determining the role of specific molecules in vivo / Rinako Nakagawa -- Characterization of the B cell transcriptome bound by RNA-binding proteins with iCLIP / Manuel D. Dıaz-Munoz, Elisa Monzon-Casanova, and Martin Turner -- Flow-cytometric method measuring B cell surface immunoglobulin avidity / Davide Angeletti, Gregory M. Frank, and Jonathan W. Yewdell -- Somatic hypermutation and affinity maturation analysis using the 4-hydroxy-3-nitrophenyl-acetyl (NP) system / Nicole Heise and Ulf Klein -- Targeting gene function in germinal center B cells : a practical approach / Valentina Petrocelli and Stefano Casola -- Development of mouse model systems of germinal center lymphomas / Eleni Kabrani and Sandrine Sander -- AID-Cre-ERT2 model : a tool for monitoring B cell immune responses and generating selective hybridomas / Simon Le Gallou, Takuya Nojima, Daisuke Kitamura, Jean-Claude Weill, and Claude-Agnès Reynaud -- Determining the origin of human germinal center B cell-derived malignancies / Marc Seifert and Ralf Kuppers -- Tracking B-cell repertoires and clonal histories in normal and malignant lymphocytes / Nicola J. Weston-Bell, Graeme Cowan, and Surinder S. Sahota -- How to simulate a germinal center / Philippe A. Robert, Ananya Rastogi, Sebastian C. Binder, and Michael Meyer-Hermann |
| Summary |
Annotation Provides key methods and protocols from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology. Written in the highly successful 'Methods in Molecular Biology' series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed June 7, 2017) |
| Subject |
Germinal centers -- Laboratory manuals
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Germinal centers.
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B cells.
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Germinal Center
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B-Lymphocytes
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Immunology.
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Medical -- Immunology.
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B cells
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Germinal centers
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Calado, Dinis Pedro, editor
|
| LC no. |
2017941511 |
| ISBN |
9781493970957 |
|
149397095X |
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1493970941 |
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9781493970940 |
|
