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Book Cover

Title PCR sequencing protocols / edited by Ralph Rapley
Published Totowa, N.J. : Humana Press, [1996]


Location Call no. Vol. Availability
 W'PONDS  572.8636 Rap/Psp  AVAILABLE
Description xi, 221 pages : illustrations ; 23 cm
Series Methods in molecular biology ; v. 65
Methods in molecular biology (Clifton, N.J.) ; v. 65
Contents Machine derived contents note: Preparation and Analysis of DNA Sequencing Gels -- Purification of PCR Products from Agarose Gels for Direct Sequencing -- Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing -- Direct Sequencing of Double-Stranded PCR Products with the Sequencase Kit and [a-35S] dATP -- Direct Sequencing by Thermal Asymmetric PCR -- Rapid Sequencing of cDNA Clones: Direct Sequencing Using Sequential Linear/Asymmetric PCR -- Direct Sequencing of PCR Products Using Chemiluminescent Detection -- Direct DNA Sequencing of PCR Products Using Magnetic Beads -- Affinity Capture and Solid-Phase Sequencing Biotinylated PCR Products -- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing. -- Nonradioactive PCR Sequencing Using Digoxygenin -- Silver SequencingTM: Nonradioactive Cycle Sequencing of dsDNA -- Direct Sequencing of PCR Products with DNA-Binding Proteins -- PCR Sequencing with the Aid of Detergents -- Direct Sequencing with Highly Degenerate and Inosine-Containing Primers -- Determination of Unknown Genomic Sequences Without Cloning -- DNA Sequencing by the Chemical Method -- Direct PCR Sequencing with Denaturants (Formamide) -- Efficient PCR Production of Single-Stranded DNA Sequencing Templates -- Preparation and Direct Automated Cycle Sequencing of PCR Products -- Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA -- Cloning PCR Products for Sequencing in M13 Vectors -- Sequencing PCR Products Cloned into M13 Vectors -- Genome Amplification with Transcript Sequencing (GAWTS) -- DNA Rescue by Vectorette Method -- Sequencing (dAidT) of Cloned Mixed PCR Products from Microbial Populations -- Index
Notes Includes index
Bibliography Includes bibliographical references and index
Notes Also available online via the World Wide Web, by subscription to (BMP) via Humana Press
Subject Polymerase chain reaction -- Laboratory manuals.
Author Rapley, Ralph.
Humana Press.
LC no. 96027724
ISBN 0896033449 (acid-free paper)