Title Differential display methods and protocols / edited by Peng Liang, Jonathan D. Meade, Arthur B. Pardee

Edition 2nd ed Published Totowa, N.J. : Humana Press, ©2006

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Description 1 online resource (xiii, 319 pages) : illustrations Series Methods in molecular biology ; v. 317 Methods in molecular biology (Clifton, N.J.) ; v. 317 Contents Methodologies -- Global analysis of gene expression by differential display: a mathematical model / Shitao Yang and Peng Liang -- Automation of fluorescent differential display with digital readout / Jonathan D. Meade [and others] -- Ordered differential display (ODD) / Mikhail V. Matz and Ella A. Meleshkevitch -- GeneCalling(tm): transcript profiling coupled to a gene database query / Richard A. Shimkets -- High density sampling differential display of prokaryotic mRNAs with RAP-PCR / Dana M. Walters and Pierre E. Rouviere -- Vertical arrays: microarrays of complex mixtures of nucleic acids / Rosana Risques [and others] -- Automated pattern ranking in differential display data analysis / Tero Aittokallio [and others] -- Linking cDNA-AFLP-based gene expression patterns and ESTs / Ling Qin, Pjotr Prins, and Johannes Helder -- Applications -- Differentially expressed genes associated with hepatitis B virus HBx and MHBs protein function in hepatocellular carcinoma / Dae-Ghon Kim -- Identification of disease markers by differential display: prion disease / Michael Clinton [and others] -- Saturation screening for p53 target genes by digital fluorescent differential display / Yong-Jig Cho [and others] -- Identification of p53-regulated genes by the method of differential display / Yunping Lin, Roger P. Leng, and Samuel Benchimol Identification by differential display of IL-24 autocrine loop activated by ras oncogenes / Zhongjia Tan, Mai Wang, and Peng Liang -- Comprehensive analysis of ovarian gene expression during ovulation using differential display / Lawrence Espey -- Functional analysis of nocturnin: a circadian clock regulated gene identified by differential display / Julie E. Baggs and Carla B. Green -- Isolation and characterization of anthocyanin 5-0-glucosyltransferase in perilla frutescens var. Crispa by differential display / Mami Yamasaki and Kazuki Saito -- Identification of target genes of a yeast transcriptional repressor / Bernard Mai and Linda L. Breeden -- Detection of an mRNA polymorphism by differential display / Shan Liang [and others] -- Silencing in yeast: identification of CLR4 targets / Sergey V. Ivanov and Alla V. Ivanova -- Identification of mRNA bound to RNA binding proteins by differential display / Anne Carr-Schmid, Xinfu Jiao, and Megerditch Kiledjian -- Differential display / Anne Carr-Schmid, Xinfu Jiao, and Megerditch Kiledjian Summary Carrying on the high standards of the first edition of Differential Display Methods, Peng Liang et al. have based their second edition on a new mathematical model of differential display (DD) that takes advantage of automation, as well as digital data acquisition and analysis. These well-versed authors explain and highlight all the recent methodological refinements, including automated liquid handling of hundreds of DD PCR reaction setups combined with capillary electrophoresis, a prototype computer program to automatically allow positive band identification from a fluorescence differential display image, and restriction fragment-based DD screenings that can link any cDNA fragment directly to a given gene once the sequence information of all transcripts becomes available. Other improvements discussed are combining DD and DNA microarrays by reducing the complexity of cDNA probes while increasing the sensitivity of detection, and a DD approach to detect prokaryotic mRNA expression. The authors also demonstrate the power of DD technology with a collection of outstanding examples of DD applications and detailed experimental procedures. The elegant studies described here have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription repression gene silencing, mRNA polymorphism, and protein-RNA interaction. State-of-the-art and highly practical, Differential Display Methods, Second Edition offers gene hunters the possibility of genome-wide comprehensive DD screening, as well as a proven road map for any successful "gene fishing" expedition Analysis moleculaire biologie molecular biology Molecular Biology (General) Moleculaire biologie (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject Messenger RNA -- Laboratory manuals Gene expression -- Research -- Methodology Polymerase chain reaction. DNA fingerprinting. Gene Expression Profiling -- methods Gene Expression -- physiology Molecular Biology -- methods Oligonucleotide Array Sequence Analysis -- methods Polymerase Chain Reaction DNA Fingerprinting SCIENCE -- Life Sciences -- Molecular Biology. DNA fingerprinting. Gene expression -- Research -- Methodology. Messenger RNA. Polymerase chain reaction. Genre/Form Laboratory manuals. Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Liang, Peng Meade, Jonathan D Pardee, Arthur B. (Arthur Beck), 1921- ISBN 9781592599684 1592599680 1280358734 9781280358739 9786610358731 6610358737 9781617375033 1617375039

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