| Description |
1 online resource |
| Contents |
Part I: Overview of Immunity -- Ch 1: Immunity -- Ch 2: Components of immunity -- Ch 3: Hematopoiesis -- Ch 4: The Immune system: pathogen sensing and detection -- Ch 5: Lymphoid organs -- Ch 6: Innate and Adaptive immunity -- Ch 7: Leukocyte homing, migration and recirculation -- Ch 8: Apoptosis -- Ch 9: Antigens -- Ch 10: B cell development, activation and immunoglobulin structure -- Ch 11: Immunoglobulin and T cell receptor gene rearrangements -- Ch 12: Major histocompatibility complex proteins -- Ch 13: T cell development and T cell receptor structure -- Ch 14: Cytokines -- Ch 15: Lymphocyte signals -- Ch 16: Complement fixation -- Ch 17: Cell mediated immunity -- Ch 18: Hypersensitivities -- Ch 19: Parasite Immunity -- Ch 20: Vaccines -- Ch 21: Transplantation -- Ch 22: Immune regulation and autoimmunity -- Ch 23: Immunodeficiencies -- Ch 24: Cancer -- Ch 25: Practice questions -- Ch 26: Study guide for exam. Part II. Laboratory Manual -- 27. Immunology laboratory policies -- 28. Instructions for notebook entries -- 29. Instructions for writing lab reports -- 30. Lab report topics -- 31. Exercise 1: Using a compound microscope -- 32. Exercise 2: Pipetting and dilution technique, identification of mouse lymphoid organs -- 33. Exercise 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing -- 34. Exercise 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens -- 35. Exercise 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells -- 36. Exercise 6: Clearance of bacteria from mouse blood by the RES -- 37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay) -- 38. EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays -- 39. EXERCISE 9: Determination of Antibody Titer: Screening Techniques -- 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis -- 41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG -- 42. EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) -- 43. EXERCISE 13: Immunoblotting (Western Blotting) -- 44. Exercise 14: Protein Assay -- 45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA) -- 46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT) -- 47. EXERCISE 17: Immunofluorescence assay (IFA) -- 48. EXERCISE 18: Flow Cytometry -- 49. EXERCISE 19: Complement Fixation Assays -- 50. Tissue Cell Culture Techniques -- 51. EXERCISE 20: Mitogen Induced Response of Lymphocytes: Detection of Band T cell Lymphocytes Using Immunofluorescence -- 52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells -- 53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production -- 54. EXERCISE 23: Macrophage Migration Inhibition -- 55. EXERCISE 24: Monoclonal Antibody Preparation -- 56. EXERCISE 25: Immunization of mice with a recombinant protein -- 57. EXERCISE 26: Polarization of T helper cells into TH1 subset -- 58. Exercise 27: Immunoprecipitation -- 59. EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers -- 60. EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies -- Appendix -- Sample Practice Questions |
| Summary |
A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed August 16, 2021) |
| Subject |
Immunology.
|
|
Immunology -- Laboratory manuals
|
|
Allergy and Immunology
|
|
Immunology
|
|
Immunologia.
|
|
Manuals de laboratori.
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
|
Llibres electrònics.
|
| Form |
Electronic book
|
| ISBN |
9783030646868 |
|
3030646866 |
|
