| Description |
1 online resource |
| Series |
Methods in molecular biology, 1064-3745 |
|
Methods in molecular biology (Clifton, N.J.)
|
| Contents |
Exploring Protein Intrinsic Disorder with MobiDB Alexander Miguel Monzon, András Hatos, Marco Necci, Damiano Piovesan, and Silvio C. E. Tosatto Part II: Evolution 7. An Easy Protocol for Evolutionary Analysis of Intrinsically Disordered Proteins Janelle Nunez-Castilla and Jessica Siltberg-Liberles Part III: Production 8. Expression and Purification of an Intrinsically Disordered Protein Karamjeet K. Singh and Steffen P. Graether 9. Production of Intrinsically Disordered Proteins for Biophysical Studies; Tips and Tricks Christian Parsbæk Pedersen, Pernille Seiffert, Inna Brakti, and Katrine Bugge 10. Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tracts and Beyond Albert Escobedo, Giulio Chiesa, and Xavier Salvatella 11. Cell-free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy Linnéa Isaksson and Anders Pedersen Part IV: Dynamics, Ensembles, and Structures 12 |
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Enhanced Molecular Dynamics Simulations of Intrinsically Disordered Proteins Matteo Masetti, Mattia Bernetti, and Andrea Cavalli 20. Computational Protocol for Determining Conformational Ensembles of Intrinsically Disordered Proteins <span style="font-family:" ms="" mincho";="" Robert B. Best 21. Computing, Analyzing and Comparing the Radius of Gyration and Hydrodynamic Radius in Conformational Ensembles of Intrinsically Disordered Proteins Mustapha Carab Ahmed, Ramon Crehuet, and Kresten Lindorff-Larsen Part VI: Determinants of Interactions 22. Binding Thermodynamics to Intrinsically Disordered Protein Domains Arne Schön and Ernesto Freire 23. Analysis of Multivalent IDP Interactions: Stoichiometry, Affinity, and Local Concentration Effect Measurements Samuel Sparks, Ryo Hayama, Michael P. Rout, and David Cowburn 24. NMR Lineshape Analysis of Intrinsically Disordered Protein Interactions Christopher A. Waudby and John Christodoulou 25 |
| Summary |
The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls |
| Notes |
Includes index |
| Subject |
Proteins -- Laboratory manuals
|
|
Proteins
|
| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
|
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Kragelund, Birthe B
|
|
Skriver, Karen
|
| ISBN |
9781071605240 |
|
1071605240 |
|
