| Description |
1 online resource (xi, 312 pages) : illustrations |
| Series |
Methods in molecular biology, 1940-6029 ; volume 2084 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; 2084. 1064-3745
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Springer protocols (Series) 1949-2448
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| Contents |
Fundamentals of Ion Mobility-Mass Spectrometry for the Analysis of Biomolecules -- Utilizing Drift Tube Ion Mobility Spectrometry for the Evaluation of Metabolites and Xenobiotics -- Untargeted Differential Metabolomics Analysis Using Drift Tube Ion Mobility-Mass Spectrometry -- Drift Tube Ion Mobility-Mass Spectrometry for Non-Targeted 'Omics -- The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines -- Traveling Wave Ion Mobility-Mass Spectrometry to Enhance the Detection of Low Abundance Features in Untargeted Lipidomics -- Lipidomics by HILIC-Ion Mobility-Mass Spectrometry -- A Workflow for the Identification of Mycotoxin Metabolites Using Liquid Chromatography?Ion Mobility-Mass Spectrometry -- Quantitation of Cyclosporin A in Cell Culture Media by Differential Mobility Mass Spectrometry (DMS-MS/MS) -- An Analytical Perspective on Protein Analysis and Discovery Proteomics by Ion Mobility-Mass Spectrometry -- Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics -- Liquid Extraction Surface Analysis (LESA) High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Mass Spectrometry for In Situ Analysis of Intact Proteins -- Ion Mobility-Mass Spectrometry of Glycoconjugates -- Desorption Atmospheric Pressure Photoionization Coupled with Ion Mobility-Mass Spectrometry -- Metabolomic Profiling of Adherent Mammalian Cells In Situ by LAESI-MS with Ion Mobility Separation -- Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled to Ion Mobility -- The Use of LipidIMMS Analyzer for Lipid Identification in Ion Mobility-Mass Spectrometry-Based Untargeted Lipidomics -- MolFind2: A Protocol for Acquiring and Integrating MS3 Data to Improve In-Silico Chemical Structure Elucidation for Metabolomics -- Collision Cross Section Calculations Using HPCCS |
| Summary |
This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Ion Mobility-Mass Spectrometry: Methods and Protocols serves as an ideal resource for scientists delving into the technique's unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed November 18, 2019) |
| Subject |
Ion mobility mass spectrometry.
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Ion mobility spectroscopy.
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Mass spectrometry.
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Ion Mobility Spectrometry -- methods
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Mass Spectrometry -- methods
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Mass Spectrometry
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mass spectrometry.
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Ion mobility mass spectrometry
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Ion mobility spectroscopy
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Mass spectrometry
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Paglia, Giuseppe (Associate professor of biochemistry), editor.
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Astarita, Giuseppe, editor
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| ISBN |
9781071600306 |
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1071600303 |
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