Title Electrophoretic separation of proteins : methods and protocols / edited by Biji T. Kurien and R. Hal Scofield

Published New York : Humana Press : Springer, [2018] ©2019

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Description 1 online resource (xv, 523 pages) : illustrations (some color) Series Methods in molecular biology ; v. 1855 Methods in molecular biology (Clifton, N.J.) ; v. 1855. 1064-3745 Contents 880-01 Intro; Preface; Contents; Contributors; Chapter 1: How It All Began: A Personal History of Gel Electrophoresis; 1 Introduction; References; Chapter 2: Introduction to Protein Electrophoresis; 1 Introduction; 2 Electrophoresis on a Solid Support; 2.1 Paper Electrophoresis; 2.2 Gel Electrophoresis; 2.2.1 Polyacrylamide Gel Electrophoresis (PAGE); 2.2.2 Buffers; 2.2.3 Two-Dimensional Gel Electrophoresis; 2.2.4 Capillary Electrophoresis; References; Chapter 3: Measuring Protein Concentration with Absorbance, Lowry, Bradford Coomassie Blue, or the Smith Bicinchoninic Acid As ... ; 1 Introduction 880-01/(S How it all began : a personal history of gel electrophoresis / Oliver Smithies -- Introduction to protein electrophoresis / Pothur R. Srinivas -- Measuring protein concentration with absorbance, lowry, bradford coomassie blue, or the smith bicinchoninic acid assay before electrophoresis / J.P. Dean Goldring -- Concentrating proteins by salt, polyethylene glycol, solvent, SDS precipitation, three-phase partitioning, dialysis, centrifugation, ultrafiltration, lyophilization, affinity chromatography, immunoprecipitation or increased temperature for protein isolation, drug interaction, and proteomic and peptidomic evaluation / J.P. Dean Goldring -- Lysis buffer choices are key considerations to ensure effective sample solubilization for protein electrophoresis / Ewa I. Miskiewicz and Daniel J. MacPhee -- Cydex blue assay : a one-step protein assay for samples prior to SDS electrophoresis / Thierry Rabilloud -- Cellulose acetate electrophoresis of hemoglobin / Ramesh Kumar and Wilbert A. Derbigny -- Native polyacrylamide gels / Claudia Arndt, Stefanie Koristka, Anja Feldmann, and Michael Bachmann -- Isoelectric focusing on non-denaturing flatbed gels / Biji T. Kurien and R. Hal Scofield -- Determination of protein molecular weights on SDS-PAGE / Hiroyuki Matsumoto, Hisao Haniu, and Naoka Komori -- Two-dimensional gel electrophoresis by glass tube-based IEF and SDS-PAGE / Hiroyuki Matsumoto, Hisao Haniu, Biji T. Kurien, and Naoka Komori -- Cationic electrophoresis / Engelbert Buxbaum -- Two-dimensional gel electrophoresis with immobilized pH gradients / Bre'Ana Byrd and Huyen Tran -- SARCOSYL-PAGE : optimized protocols for the separation and immunologicaldDetection of PEGylated proteins / Christian Reichel, Gu·nter Gmeiner, Philipp Reihlen, Mario Thevis, and Wilhelm Sch€anzer -- Tricine-SDS-PAGE / Syed R. Haider, Helen J. Reid, and Barry L. Sharp -- Analysis of protein glycation using phenylboronate acrylamide gel electrophoresis / Marta P. Pereira Morais, Omar Kassaar, Stephen E. Flower, Robert J. Williams, Tony D. James, and Jean M.H. van den Elsen -- Immunofixation electrophoresis for identification of proteins and specific antibodies / Gyorgy Csako -- Electrophoretic separation of very large molecular weight proteins in SDS agarose / Marion L. Greaser and Chad M. Warren -- Increase in local protein concentration by field-inversion gel electrophoresis / Henghang Tsai and Hon-Chiu Eastwood Leung -- Two-dimensional difference gel electrophoresis / Malachi Blundon, Vinitha Ganesan, Brendan Redler, Phu T. Van, and Jonathan S. Minden -- "Microchip Electrophoresis," with respect to "Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease" / Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy -- Measuring low-picomolar apparent binding affinities by minigel electrophoretic mobility shift / Karen A. Lewis, Sarah E. Altschuler, and Deborah S. Wuttke -- Identification of proteins interacting with single nucleotide polymorphisms (SNPs) by DNA pull-down assay / Bhupinder Singh and Swapan K. Nath -- Horizontal agarose gel mobility shift assay for protein-RNA complexes / Jennifer A. Ream, L. Kevin Lewis, and Karen A. Lewis -- Applications of immobilized metal affinity electrophoresis / Bao-Shiang Lee, Lasanthi P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta -- Isoelectric focusing in agarose gel for detection of oligoclonal bands in cerebrospinal and other biological fluids / Gyorgy Csako -- Combined free-flow electrophoresis and DIGE approach to compare proteins in complex biological samples / Kim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann -- SDS-PAGE for 35S immunoprecipitation and immunoprecipitation western blotting / Edward P. Trieu and Ira N. Targoff -- Multichannel gel electrophoresis and continuous fraction collection apparatus for high-throughput protein separation and characterization / Ming Dong, Megan Choi, Mark D. Biggin, and Jian Jin -- Cell surface protein biotinylation for SDS-PAGE analysis / Giuliano Elia -- Isolation of proteins from polyacrylamide gel / Stefanie Koristka, Claudia Arndt, Ralf Bergmann, and Michael Bachmann -- Continuous elution SDS-PAGE with a modified standard gel apparatus to separate and isolate an array of proteins from complex mixtures / Robert G.E. Krause and J.P. Dean Goldring -- Protein extraction from gels : a brief review / Biji T. Kurien, Rachna Aggarwal, and R. Hal Scofield -- Gel absorption-based sample preparation method for shotgun analysis of membrane proteome / Xianchun Wang and Songping Liang -- Ultrarapid sodium dodecyl sulfate polyacrylamide mini-gel electrophoresis / Rohit Thomas and Biji T. Kurien -- Brief review of other notable electrophoretic methods / James D. Fesmire -- Single-cell high-resolution detection and quantification of protein isoforms differing by a single charge unit / Kristi A. Koelsch -- Artifacts and common errors in protein gel electrophoresis / Biji T. Kurien and R. Hal Scofield 1.1 Detecting Proteins with Absorbance at 280 nm1.2 Detecting Proteins with the Lowry Method; 1.3 Detecting Proteins with the Smith Bicinchoninic Acid Method; 1.4 Detecting Proteins with the Bradford Coomassie Blue G-250 Method; 2 Materials; 2.1 Detecting Proteins by Measuring Absorbance at 280 nm or Using a Standard Curve of Absorbance Against Protein Concentration ... ; 2.2 Detecting Proteins with the Lowry Method; 2.3 Detecting Proteins with the Smith Bicinchoninic Acid Method; 2.4 Detecting Proteins with the Bradford Coomassie Blue G-250 Method; 3 Methods 3.1 Detecting Proteins by Measuring Absorbance at 280 nm3.2 Detecting Proteins Using a Standard Curve of Protein Against Absorbance at 280 nm; 3.3 Detecting Proteins with the Lowry Method; 3.4 Detecting Proteins with the Smith Bicinchoninic Acid Method; 3.5 Detecting Proteins with the Bradford Coomassie Blue G-250 Method; 4 Notes; References; Chapter 4: Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three-Phase Partitioning, Dialysis ... ; 1 Introduction; 1.1 Concentrating Proteins by Precipitation with Salts and Polyethylene Glycol 1.2 Concentrating Proteins by Precipitation with Organic Solvents and Trichloroacetic Acid (TCA)1.3 Concentrating Proteins for SDS-PAGE with SDS/KCl Precipitation; 1.4 Concentrating Proteins with Three-Phase Partitioning; 1.5 Concentrating Protein by the Absorption of Solvent; 1.6 Centrifugation to Concentrate Proteins; 1.7 Concentrating Proteins with Increased Pressure; 1.8 Concentrating Protein Solutions Using a Vacuum; 1.9 Concentrating Protein Solutions Using a Vacuum and Freezing: Freeze-Drying (Lyophilization); 1.10 Concentrating Proteins Using Affinity Chromatography 1.11 Concentrating Proteins with Antibodies in Immunoprecipitation Assays1.12 Concentrating Proteins Using Thermal Denaturation; 1.13 Concentrating Protein Solutions for Proteomic and Peptidomic Studies; 2 Materials; 2.1 Concentrating Proteins by Precipitation with Ammonium Sulfate and Polyethylene Glycol; 2.2 Concentrating Proteins by Precipitation with Organic Solvents and Trichloroacetic Acid (TCA); 2.3 Concentrating Proteins for SDS-PAGE with SDS/KCl Precipitation; 2.4 Concentrating Proteins with Three-Phase Partitioning; 2.5 Concentrating Protein by the Absorption of Solvent Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed November 13, 2018) Subject Electrophoresis -- Laboratory manuals Proteins -- Separation -- Laboratory manuals Gel electrophoresis. Immunoelectrophoresis. Proteins. Electrochemical Techniques Electrophoresis -- methods Chemistry Techniques, Analytical Electrophoresis, Agar Gel Immunoelectrophoresis Proteins Laboratory Manuals protein. Proteins. Science -- Life Sciences -- Biochemistry. Proteins Immunoelectrophoresis Gel electrophoresis Electrophoresis Proteins -- Separation Genre/Form Electronic books Laboratory manuals Form Electronic book Author Kurien, Biji T., editor. Scofield, R. Hal, editor. ISBN 9781493987931 1493987933

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