Title Site-specific protein labeling : methods and protocols / edited by Arnaud Gautier, Marlon J. Hinner

Published New York, NY : Humana Press : Springer, [2015] ©2015

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Description 1 online resource (xii, 267 pages) : illustrations (some color) Series Methods in Molecular Biology, 1940-6029 ; 1266 Methods in molecular biology (Clifton, N.J.) ; v. 1266. 1064-3745 Contents How FlAsH got its dparkle : historical recollections of the biarsenical-tetracysteine tag / B. Albert Griffin, Stephen R. Adams, and Roger Y. Tsien -- Site-specific protein labeling in the pharmaceutical industry : experiences from novartis drug discovery / Lukas Leder -- Getting across the cell membrane : an overview for small molecules, peptides, and proteins / Nicole J. Yang and Marlon J. Hinner -- Considerations and protocols for the synthesis of custom protein labeling probes / Ivan R. Corrêa Jr. -- 2-Cyanobenzothiazole (CBT) condensation for site- specific labeling of proteins at the terminal cysteine residues / Lina Cui and Jianghong Rao -- Fluorescent labeling for patch-clamp fluorometry (PCF) measurements of real-time protein motion in ion channels / Thomas K. Berger and Ehud Y. Isacoff -- Fluorescent labeling of SNAP-tagged proteins in cells / Gražvydas Lukinavičius, Luc Reymond, and Kai Johnsson -- Halotag technology for specific and covalent labeling of fusion proteins / Hélène A. Benink and Marjeta Urh -- Ligation of synthetic peptides to proteins using semisynthetic protein trans-splicing / Julian C.J. Matern [and others] -- Chemical-tag labeling of proteins using fully recombinant split inteins / Anne-Lena Bachmann [and others] -- Phage selection assisted by Sfp phosphopantetheinyl transferase-catalyzed site-specific protein labeling / Bo Zhao [and others] -- Site-specific biotinylation of purified proteins using BirA / Michael Fairhead and Mark Howarth -- Site-specific labeling of proteins via sortase : protocols for the molecular biologist / Maximilian Wei-Lin Popp -- BONCAT: metabolic labeling, click chemistry, and affinity purification of newly synthesized proteomes / Peter Landgraf, Elmer R. Antileo, Erin M. Schuman, and Daniela C. Dieterich -- Genetic encoding of unnatural amino acids for labeling proteins / Kathrin Lang, Lloyd Davis, and Jason W. Chin -- Labeling proteins by affinity-guided DMAP chemistry / Tomonori Tamura and Itaru Hamachi -- Ligand-directed tosyl chemistry for selective native protein labeling in vitro, in cells, and in vivo / Shinya Tsukiji and Itaru Hamachi Summary This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, "Site-Specific Protein Labeling: Methods and Protocols" provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question Analysis eiwitten proteins Proteins and Enzymes Eiwitten en enzymen Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (SpringerLink, viewed January 23, 2015) Subject Proteins -- Analysis -- Laboratory manuals Proteins. Methodology. Stains and staining (Microscopy) Cytology -- Technique. Histology -- Technique. Medical laboratory technology. Proteins Methods Staining and Labeling Laboratory Manuals Amino Acids, Peptides, and Proteins Investigative Techniques Histocytological Preparation Techniques Publication Formats Publication Characteristics Chemicals and Drugs Cytological Techniques Histological Techniques Analytical, Diagnostic and Therapeutic Techniques and Equipment Clinical Laboratory Techniques protein. methodology. staining (testing technique) stains (damage) stain (coating) Proteins. Science -- Life Sciences -- Biochemistry. Stains and staining (Microscopy) Proteins Methodology Medical laboratory technology Histology -- Technique Cytology -- Technique Proteins -- Analysis Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Gautier, Arnaud, editor. Hinner, Marlon J., editor. ISBN 9781493922727 1493922726 9781493921720 149392172X

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