| Description |
1 online resource (xi, 267 pages) : illustrations (some color) |
| Series |
Springer protocols |
|
Methods in molecular biology, 1064-3745 ; 592 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 592
|
| Contents |
Piecing the puzzle together : genetic requirements for miRNA biogenesis in Arabidopsis thaliana / Zhixin Xie -- Prediction of plant miRNA genes / Matthew W. Jones-Rhoades -- Methods for isolation of total RNA to recover miRNAs and other small RNAs from diverse species / Monica Accerbi [and others] -- miRNA target prediction in plants / Noah Fahlgren and James C. Carrington -- A method to discover phased siRNA loci / Michael J. Axtell -- Directed gene silencing with artificial microRNAs / Rebecca Schwab [and others] -- Bioinformatics analysis of small RNAs in plants using next generation sequencing technologies / Kan Nobuta [and others] -- High-throughput approaches for miRNA expression analysis / Cheng Lu and Frédéric Souret -- In situ detection of miRNAs using LNA probes / Zoltán Havelda -- Analysis of miRNA modifications / Bin Yu and Xuemei Chen -- MicroRNA promoter analysis / Molly Megraw and Artemis G. Hatzigeorgiou -- Computational methods for comparative analysis of plant small RNAs / Gayathri Mahalingam and Blake C. Meyers -- Biotic stress-associated microRNAs : identification, detection, regulation, and functional analysis / Florence Jay [and others] -- Abiotic stress-associated miRNAs : detection and functional analysis / Dong-Hoon Jeong [and others] -- Processing of miRNA precursors / Yukio Kurihara and Yuichiro Watanabe -- Purification of Arabidopsis argonaute complexes and associated small RNAs / Yijun Qi and Shijun Mi -- Transient assays for the analysis of miRNA processing and function / Felipe F. de Felippes and Detlef Weigel |
| Summary |
MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike |
| Analysis |
planten |
|
plants |
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rna |
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identificatie |
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identification |
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karakterisering |
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characterization |
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fylogenie |
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phylogeny |
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nucleotidenvolgordes |
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nucleotide sequences |
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moleculaire biologie |
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molecular biology |
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biologische technieken |
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biological techniques |
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laboratoriummethoden |
|
laboratory methods |
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transcriptomics |
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protocollen |
|
protocols |
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Plant Molecular Biology |
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Moleculaire plantenbiologie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Small interfering RNA.
|
|
Plant molecular genetics -- Research -- Methodology
|
|
MicroRNA.
|
|
MicroRNAs
|
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RNA, Small Interfering
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Plants -- genetics
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MicroRNA
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Small interfering RNA
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| Genre/Form |
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Meyers, Blake C.
|
|
Green, Pamela J.
|
| ISBN |
9781603270052 |
|
1603270051 |
|
