| Description |
1 online resource (xii, 442 pages) : illustrations |
| Contents |
Front Cover -- PRINCIPLES OF NUCLEIC ACID STRUCTURE -- PRINCIPLES OF NUCLEIC ACID STRUCTURE -- Copyright -- CONTENTS -- Preface to the second edition -- Preface from the first edition -- 1 -- Methods for studying nucleic acid structure -- 1.1 Introduction -- 1.2 X-ray diffraction methods for structural analysis -- 1.2.1 Overview -- 1.2.2 Fibre diffraction methods -- 1.2.3 Single-crystal methods -- 1.3 NMR methods for studying nucleic acid structure and dynamics -- 1.4 Cryo-EM methods for macromolecular complexes -- 1.5 Molecular modelling and simulation of nucleic acids -- 1.6 Chemical, enzymatic and biophysical probes of structure and dynamics -- 1.7 Sources of structural data -- 1.8 Visualisation of nucleic acid molecular structures -- 1.8.1 The structural visualisations in this book -- References -- Further reading -- General -- The history of DNA structure -- More detailed accounts of particular topics can be found in the following -- X-ray crystallography -- NMR methods -- Molecular modelling and simulation methods -- Chemical, enzymatic, biophysical and single-molecule probes of nucleic acid structure -- The nucleic acid and protein databases -- 2 -- The building blocks of DNA and RNA -- 2.1 Introduction -- 2.2 Base pairing -- 2.3 Base and base pair flexibility -- 2.4 Sugar puckers -- 2.5 Conformations about the glycosidic bond -- 2.6 The backbone torsion angles and correlated flexibility -- References -- Further reading -- 3 -- DNA structure as observed in fibres and crystals -- 3.1 Structural fundamentals -- 3.1.1 Helical parameters -- 3.1.2 Base-pair morphological features -- 3.2 Polynucleotide structures from fibre diffraction studies -- 3.2.1 Classic DNA structures -- 3.2.2 DNA polymorphism in fibres -- 3.3 B-DNA oligonucleotide structure as seen in crystallographic analyses -- 3.3.1 The Dickerson-Drew dodecamer |
|
3.3.2 Other studies of the Dickerson-Drew dodecamer -- 3.3.3 Other B-DNA oligonucleotide structures -- 3.3.4 Sequence-dependent features of B-DNA -- their occurrence and their prediction -- 3.4 A-DNA oligonucleotide crystal structures -- 3.4.1 A-form octanucleotides -- 3.4.2 Do A-form oligonucleotides occur in solution? Crystal packing effects -- 3.4.3 The A↔B transition in crystals -- 3.5 Z-DNA -- left-handed DNA -- 3.5.1 The Z-DNA hexanucleotide crystal structure -- 3.5.2 Overall structural features -- 3.5.3 The Z-DNA helix -- 3.5.4 Other Z-DNA structures -- 3.5.5 Biological aspects of Z-DNA -- 3.6 Bent DNA -- 3.6.1 DNA periodicity in solution -- 3.6.2 A-tracts and bending -- 3.6.3 Structures showing bending -- 3.6.4 The structure of poly dAdT -- 3.7 Concluding remarks -- References -- Further reading -- 4 -- Non-standard and higher-order DNA structures: DNA-DNA recognition -- 4.1 Mismatches in DNA -- 4.1.1 General features -- 4.1.2 Purine:purine mis-matches -- 4.1.3 Alkylation mismatches -- 4.1.4 Alkylation at Watson-Crick base pairs -- 4.2 DNA triple helices -- 4.2.1 Introduction -- 4.2.2 Structural studies -- 4.2.3 Anti-parallel triplexes and non-standard base pairings -- 4.2.4 Triplex applications -- 4.3 Guanine quadruplexes -- 4.3.1 Introduction -- 4.3.2 Overall structural features of quadruplex DNA -- 4.3.3 Telomeric quadruplex structures -- 4.3.4 Human telomeric quadruplex structures -- 4.3.5 Promoter and other quadruplexes -- 4.3.6 Quadruplex-duplex junctions -- 4.3.7 Left-handed quadruplexes -- 4.4 The i-motif -- 4.5 DNA junctions -- 4.5.1 Holliday junction structures -- 4.5.2 DNA enzyme structures -- 4.6 Unnatural DNA structures -- References -- Further reading -- Mispairing -- Triplexes -- Telomeres and quadruplexes -- DNA junctions -- 5 -- Principles of small molecule-DNA recognition -- 5.1 Introduction -- 5.2 DNA-water interactions |
|
7.6.3 The OB-fold domain -- 7.6.4 The immunoglobulin fold -- 7.6.5 B3 domains -- 7.7 Minor groove recognition -- 7.7.1 Recognition of B-DNA -- 7.7.2 The opening-up of the minor groove by TBP -- 7.7.3 Other proteins that induce bending of DNA -- 7.8 DNA bending and protein recognition -- 7.9 Protein-DNA small-molecule recognition -- 7.10 DNA-binding proteins and their role in genomics -- 7.10.1 Zinc finger proteins -- 7.10.2 The development of zinc finger targeting -- 7.10.3 Repair of DNA lesions by site-specific cutting and DNA recombination -- 7.10.4 The regulation of gene control by ZFPs -- 7.10.5 CRISPR-associated nuclease Cas9 -- References -- Further reading -- Useful web sites -- 8 -- RNA binding proteins -- 8.1 Aminoacyl synthetase-tRNA complexes -- 8.2 Structure of reverse transcriptases -- 8.3 snRPs (small nuclear ribonucleoproteins) -- 8.4 The ribosome -- 8.4.1 The ribosomal subunits -- 8.4.1.1 The 30S subunit -- 8.4.1.2 The 50S subunit -- 8.4.1.3 The entire 70S ribosomal structure -- 8.4.2 Other protein GTPases acting in the ribosomal cycle -- 8.4.2.1 EF-G -- 8.4.3 Further ribosome studies -- 8.4.4 Eukaryotic ribosomes -- References -- tRNA-synthetases -- bksec2_5 -- Reverse Transcriptases (RT) -- bksec2_6 -- snRPs -- bksec2_7 -- The Ribosome -- bksec2_8 -- Further reading -- Books -- Index -- Back Cover |
| Summary |
Principles of Nucleic Acid Structure, Second Edition, provides the most complete and concise summary of underlying principles and approaches to studying nucleic acid structure, including discussions of X-ray crystallography, NMR, molecular modelling and databases. The book's focus is on a survey of structures that are especially important for biomedical research and pharmacological applications. This updated edition includes the latest advances relevant to recognition of DNA and RNA by small molecules and proteins, including sections on RNA folding, ribosome structure and antibiotic interactions, DNA quadruplexes, DNA and RNA protein complexes and short interfering RNA (siRNA). This reference is a must-have for those seeking an authoritative, comprehensive and up-to-date source on all aspects of nucleic acid structure, from basic first principles to details of recent research results |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (ScienceDirect, viewed November 1, 2021) |
| Subject |
Nucleic acids -- Structure
|
|
Nucleic acids -- Structure
|
| Form |
Electronic book
|
| Author |
Sanderson, Mark, author
|
| ISBN |
9780128196786 |
|
0128196785 |
|
