| Description |
1 online resource (xv, 335 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; v. 456 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 456.
|
| Contents |
Overview of adipose tissue and its role in obesity and metabolic disorders / Gema Frühbeck -- Choosing an adipose tissue depot for sampling / Louis Casteila [and others] -- Application of imaging and other noninvasive techniques in determining adipose tissue mass / Wei Shen, Jun Chen -- Generation of adipose tissue-specific transgenic mouse models / Xian-Cheng Jiang -- Angiogenesis in adipose tissue / Ebba Brakenhielm, Yihai Cao -- Adipose organ nerves revealed by immunohistochemistry / Antonio Giordano, Andrea Frontini, Saverio Cinti -- Human adipose tissue blood flow and micromanipulation of human subcutaneous blood flow / Gijs H. Goossens, Fredrik Karpe -- Studies of thermogenesis and mitochondrial function in adipose tissues / Barbara Cannon, Jan Nedergaard -- Application of lipidomics and metabolomics to the study of adipose tissue / Ismo Mattila [and others] -- Applications of proteomics to the study of adipose tissue / Sonja Hess, Xiaoli Chen -- Application of DNA microarray to the study of human adipose tissue/cells / Paska A. Permana, Saraswathy Nair, Yong-Ho Lee -- Differentiation of adipose stem cells / Bruce A. Bunnell [and others] -- Methods that resolve different contributions of clonal expansion to adiposgenesis in 3T3-L1 and C3H10T1/2 cells / Colin R. Jefcoate, Suqing Wang, Xueqing Liu -- Explant cultures of white adipose tissue / Sebastien Thalmann, Cristiana E. Juge-Aubry, Christopher A. Meier |
|
Isolation and culture of preadipocytes from rodent white adipose tissue / Dorothy B. Hausman, Hea Jin Park, Gary J. Hausman -- Flow cytometry on the stromal-vascular fraction of white adipose tissue / Danett K. Brake, C. Wayne Smith -- Application of electrophoretic mobility shift assay and chromatin immunoprecipitation in the study of transcription in adipose cells / Melina M. Musri, Ramon Gomis, Marcelina Párrizas -- Application of RNA interference techniques to adipose cell cultures / Shigeki Shimba -- RNA isolation and real-time quantitative RT-PCR / Haiyan Guan, Kaiping Yang -- Study of adipose tissue gene expression by In Situ hybridization / Michel Grino -- Application of immunocytochemistry and immunofluorescence techniques to adipose tissue and cell cultures / Daniela Malide -- Determination of lipolysis in isolated primary adipocytes / Srikant Viswanadha, Constantine Londos -- Study of glucose uptake in adipose cells / Jun Shi, Konstantin V. Kandror -- Measurement of phosphoinositide 3-kinase and its products to study adipogenic signal transduction / Alexander Sorisky, AnneMarie Gagnon |
| Summary |
Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years |
| Analysis |
vetcellen |
|
adipocytes |
|
vetweefsel |
|
adipose tissue |
|
obesitas |
|
obesity |
|
stofwisselingsstoornissen |
|
metabolic disorders |
|
pathogenese |
|
pathogenesis |
|
laboratoriummethoden |
|
laboratory methods |
|
protocollen |
|
protocols |
|
Biological Techniques |
|
Molecular Biology (General) |
|
Biologische technieken |
|
Moleculaire biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Adipose tissues.
|
|
Adipocytes -- physiology
|
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Adipose Tissue -- physiology
|
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Tissue Culture Techniques -- methods
|
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Adipose Tissue
|
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Adipose tissues
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Yang, Kaiping
|
| ISBN |
9781597452458 |
|
1597452459 |
|
9781617378447 |
|
1617378445 |
|
