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Title Wound regeneration : methods and protocols / Hiranmoy Das
Published New York, NY : Humana Press, [2021]

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Description 1 online resource (1 PDF file (xi, 207 pages)) : illustrations
Series Methods in molecular biology, 1940-6029 ; 2193
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 2193. 1064-3745
Springer protocols (Series) 1949-2448
Contents Preface -- Chapter 1. Investigating Epidermal Interactions Through an In Vivo Cutaneous Wound-Healing Assay -- Chapter 2. A Murine Incisional Fetal Wound-Healing Model to Study Scarless and Fibrotic Skin Repair -- Chapter 3. Development of Cutaneous Wound in Diabetic Immunocompromised Mice and Use of Dental Pulp-Derived Stem Cell Product for Healing -- Chapter 4. Musculoskeletal Tissue Engineering Using Fibrous Biomaterials -- Chapter 5. Cutaneous Wound Generation in Diabetic NOD/SCID Mice and the Use of Nanofiber-Expanded Hematopoietic Stem Cell Therapy -- Chapter 6. Contusion Rodent Model of Traumatic Brain Injury : Controlled Cortical Impact -- Chapter 7. Evaluation of MicroRNA Therapeutic Potential Using the Mouse In Vivo and Human Ex Vivo Wound Models -- Chapter 8. Cellular Migration Assay : An In Vitro Technique to Simulate the Wound Repair Mechanism -- Chapter 9. In Vivo Ear Sponge Lymphangiogenesis Assay -- Chapter 10. Identification of Rho GEF and RhoA Activation by Pull-Down Assays -- Chapter 11. Wound Matrix Stiffness Imposes on Macrophage Activation -- Chapter 12. Generation of Acute Hind Limb Ischemia in NOD/SCID Mice and Treatment with Nanofiber-Expanded CD34+ Hematopoietic Stem Cells -- Chapter 13. Electrospun Aligned Coaxial Nanofibrous Scaffold for Cardiac Repair -- Chapter 14. Generation of Myocardial Ischemic Wounds and Healing with Stem Cells -- Chapter 15. Corneal Repair Models in Mice : Epithelial/Mechanical Versus Stromal/Chemical Injuries -- Chapter 16. Topical Adoptive Transfer of Plasmacytoid Dendritic Cells for Corneal Wound Healing -- Chapter 17. Murine Corneal Epithelial Wound Modeling -- Chapter 18. Infection-Induced Porcine Ex Vivo Corneal Wound Model to Study the Efficacy of Herpes Simplex Virus-1 Entry and Replication Inhibitors
Summary This detailed book explores a diverse range of topics related to wound healing. Some areas include wound generation as a tool to study cellular interactions and growth, mechanisms by which different tissues regenerate, ischemic wounds and their treatment, repairing corneal wound, as well as a number of emerging technologies to help promote wound regeneration, such as techniques with miRNA, nanomaterials, biomaterials, and stem cell therapies. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Wound Regeneration: Methods and Protocols serves as a collection of wound model development and regeneration studies
Bibliography Includes bibliographical references and index
Notes 1. Investigating Epidermal Interactions through an In Vivo Cutaneous Wound Healing Assay John L. Zemkewicz, Racheal G. Akwii, Constantinos M. Mikelis, and Colleen L. Doçi 2. A Murine Incisional Fetal Wound Healing Model to Study Scarless and Fibrotic Skin Repair Traci A. Wilgus 3. Development of Cutaneous Wound in Diabetic Immunocompromised Mice and Use of Dental Pulp-Derived Stem Cell Product for Healing Carl Greene and Hiranmoy Das 4. Musculoskeletal Tissue Engineering Using Fibrous Biomaterials George Tan, Yingge Zhou, and Dilshan Sooriyaarachchi 5. Cutaneous Wound Generation in Diabetic NOD/SCID Mice and the Use of Nanofiber-Expanded Hematopoietic Stem Cell Therapy Sarah Anderson and Hiranmoy Das 6. Contusion Rodent Model of Traumatic Brain Injury: Controlled Cortical Impact Marie-Line Fournier, Tifenn Clément, Justine Aussudre, Nikolaus Plesnila, André Obenaus, and Jérôme Badaut 7
Evaluation of MicroRNA Therapeutic Potential Using the Mouse In Vivo and Human Ex Vivo Wound Models Xi Li and Ning Xu Landén 8. Cellular Migration Assay: An In Vitro Technique to Simulate the Wound Repair Mechanism A.K.M. Nawshad Hossian and George Mattheolabakis 9. In Vivo Ear Sponge Lymphangiogenesis Assay Racheal G. Akwii, Md S. Sajib, Fatema T. Zahra, Hanumantha R. Madala, Kalkunte S. Srivenugopal, and Constantinos M. Mikelis 10. Identification of Rho-GEF and RhoA Activation by Pull-Down Assays Md S. Sajib, Fatema T. Zahra, Racheal G. Akwii, and Constantinos M. Mikelis 11. Wound Matrix Stiffness Imposes on Macrophage Activation Pu Duann and Pei-Hui Lin 12. Generation of Acute Hind Limb Ischemia in NOD/SCID Mice and Treatment with Nanofiber-Expanded CD34+ Hematopoietic Stem Cells Derek Barthels and Hiranmoy Das 13. Electrospun Aligned Co-Axial Nanofibrous Scaffold for Cardiac Repair Divya Sridharan, Arunkumar Palaniappan, Britani N. Blackstone, Heather Powell, and Mahmood Khan 14
Generation of Myocardial Ischemic Wounds and Healing with Stem Cells Daniela Rolph and Hiranmoy Das 15. Corneal Repair Models in Mice: Epithelial/Mechanical Versus Stromal/Chemical Injuries Peipei Pan and Matilda F. Chan 16. Topical Adoptive Transfer of Plasmacytoid Dendritic Cells for Corneal Wound Healing Arsia Jamali, Brendan M. Kenyon, Gustavo Ortiz, Betul N. Bayraktutar, Victor G. Sendra, and Pedram Hamrah 17. Murine Corneal Epithelial Wound Modeling Dhara Shah and Vinay Kumar Aakalu 18. Infection-Induced Porcine Ex Vivo Corneal Wound Model to Study the Efficacy of Herpes Simplex Virus-1 Entry and Replication Inhibitors Tejabhiram Yadavalli, Raghuram Koganti, and Deepak Shukla
Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL
Description based on online resource; title from PDF title page (viewed Sept. 8, 2021)
Subject Life sciences.
Regenerative medicine.
Tissue engineering.
Stem cells.
Wound healing.
Regeneration (Biology)
Wound Healing
Regeneration
Tissue Engineering -- methods
Biological Science Disciplines
Regenerative Medicine
Tissue Engineering
Stem Cells
biological sciences.
Biology, life sciences.
Genetics (non-medical).
Cellular biology (cytology).
Science -- Life Sciences -- Biology -- General.
Science -- Life Sciences -- Cytology.
Science -- Life Sciences -- Biology -- Molecular Biology.
Wound healing
Regeneration (Biology)
Life sciences
Regenerative medicine
Stem cells
Tissue engineering
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Das, Hiranmoy, editor
ISBN 9781071608456
1071608452