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Book Cover
Author Butler, John M. (John Marshall), 1969-

Title Advanced topics in forensic DNA typing : methodology / John M. Butler
Published San Diego, CA : Elsevier Academic Press, ©2012
Online access available from:
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Description 1 online resource
Contents 1. Sample Collection, Storage, and Characterization -- 2. DNA Extraction Methods -- 3. DNA Quantitation -- 4. PCR Amplification: Capabilities and Cautions -- 5. Short Tandem Repeat (STR) Loci and Kits -- 6. Capillary Electrophoresis: Principles and Instrumentation -- 7. Quality Assurance and Validation -- 8. DNA Databases: Uses and Issues -- 9. Missing Persons and Disaster Victim Identification Efforts -- 10. Degraded DNA -- 11. Low-Level DNA Testing: Issues, Concerns, and Solutions -- 12. Single Nucleotide Polymorphisms and Applications -- 13. Y-Chromosome DNA Testing -- 14. Mitochondrial DNA Analysis -- 15. X-Chromosome Analysis -- 16. Non-Human DNA -- 17. New Technologies and Automation -- 18. Legal Aspects of DNA Testing and the Scientific Expert in Court -- Appendix 1 Reported Sizes and Sequences of STR Alleles -- Appendix 2 Familial DNA Searches: Potential, Pitfalls, and Privacy Concerns -- Appendix 3 List of Suppliers for DNA Instruments, Reagents, Services -- Appendix 4 Interviews Supporting Legal Aspects of DNA Testing
Sample collection, storage, and characterization -- DNA extraction methods -- DNA quantitation -- PCR amplification: capabilities and cautions -- Short tandem repeat (STR) loci and kits -- Capillary electrophoresis: principles and instrumentation -- Quality assurance and validation -- DNA databases: uses and issues -- Missing persons and disaster victim identification efforts -- Degraded DNA -- Low-level DNA testing: issues, concerns, and solutions -- Single nucleotide polymorphisms and applications -- Y-chromosome DNA testing -- Mitochondrial DNA analysis -- X-chromosome analysis -- Non-human DNA -- New technologies and automation -- Legal aspects of DNA testing and the scientific expert in court
Summary Intended as a companion to the Fundamentals of Forensic DNA Typing volume published in 2009, Advanced Topics in Forensic DNA Typing: Methodology contains 18 chapters with 4 appendices providing up-to-date coverage of essential topics in this important field and citation to more than 2800 articles and internet resources. The book builds upon the previous two editions of John Butler's internationally acclaimed Forensic DNA Typing textbook with forensic DNA analysts as its primary audience. This book provides the most detailed information written to-date on DNA databases, low-level DNA, validation, and numerous other topics including a new chapter on legal aspects of DNA testing to prepare scientists for expert witness testimony. Over half of the content is new compared to previous editions. A forthcoming companion volume will cover interpretation issues. Contains the latest information - hot-topics and new technologies Well edited, attractively laid out, and makes productive use of its four-color format Author John Butler is ranked as the number one "high-impact author in legal medicine and forensic science, 2001 to 2011" by
Bibliography Includes bibliographical references and index
Notes Print version record
Subject DNA fingerprinting.
Forensic genetics.
DNA Fingerprinting -- methods
Forensic Genetics -- methods
DNA -- physiology
DNA Fingerprinting
MEDICAL -- Forensic Medicine.
DNA fingerprinting
Forensic genetics
Genre/Form Electronic book
Form Electronic book
ISBN 9780123878236