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Title Tandem repeats in genes, proteins, and disease : methods and protocols / edited by Danny M. Hatters, Anthony J. Hannan
Published New York : Humana Press, ©2013

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Description 1 online resource (xi, 258 pages) : illustrations
Series Methods in molecular biology, 1940-6029 ; 1017
Methods in molecular biology (Clifton, N.J.) ; v. 1017. 1064-3745
Contents Longitudinal imaging and analysis of neurons expressing polyglutamine-expanded proteins / Andrey S. Tsvetkov, D. Michael Ando, and Steven Finkbeiner -- Atomic force microscopy assays for evaluating polyglutamine aggregation in solution and on surfaces / Kathleen A. Burke and Justin Legleiter -- Morphometric analysis of Huntington's disease neurodegeneration in Drosophila / Wan Song [and others] -- Size analysis of polyglutamine protein aggregates using fluorescence detection in an analytical ultracentrifuge / Saskia Polling, Danny M. Hatters, and Yee-Foong Mok -- Method for the incremental expansion of polyglutamine repeats in recombinant proteins / Amy L. Robertson and Stephen P. Bottomley -- Pulse shape analysis (PulSA) to track protein translocalization in cells by flow cytometry : applications for polyglutamine aggregation / Yasmin M. Ramdzan, Rebecca Wood, and Danny M. Hatters -- Characterizing social behavior in genetically targeted mouse models of brain disorders / Emma L. Burrows and Anthony J. Hannan -- PCR amplification and sequence analysis of GC-rich sequences : aristaless-related homeobox example / May H. Tan, Jozef Gécz, and Cheryl Shoubridge -- Challenges of "Sticky" co-immunoprecipitation : polyalanine tract protein-protein interactions / T.R. Mattiske [and others] -- Molecular pathology of polyalanine expansion disorders : new perspectives from mouse models / James N. Hughes and Paul Q. Thomas -- Yeast as a platform to explore polyglutamine toxicity and aggregation / Martin L. Duennwald -- Immuno-based detection assays to quantify distinct mutant huntingtin conformations in biological samples / Gregor P. Lotz and Andreas Weiss -- Modeling and analysis of repeat RNA toxicity in Drosophila / S.E. Samaraweera [and others] -- Analyzing modifiers of protein aggregation in C. elegans by native agarose gel electrophoresis / Mats Holmberg and Ellen A.A. Nollen -- Kinetic analysis of aggregation data / Regina M. Murphy -- Bioinformatics method for identifying Q/N-rich prion-like domains in proteins / Eric D. Ross [and others] -- Detecting soluble polyQ oligomers and investigating their impact on living cells using split-GFP / Patrick Lajoie and Erik Lee Snapp -- Cell biological approaches to investigate polyglutamine-expanded AR metabolism / Lori J. Cooper and Diane E. Merry
Summary The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences. Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine. Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states. This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences
Analysis Medicine
Human genetics
Medical genetics
Biomedicine
Gene Function
biomedische wetenschappen
moleculaire genetica
molecular genetics
humane genetica
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Subject Nucleotides -- Laboratory manuals
Genetics -- Laboratory manuals
Biology.
Genomes.
Life sciences.
Physical sciences.
Molecular structure.
Diseases.
Publication Formats
Repetitive Sequences, Nucleic Acid
Biology
Genome Components
Pathologic Processes
Phenomena and Processes
Genome
Pathological Conditions, Signs and Symptoms
Base Sequence
Publication Characteristics
Biological Science Disciplines
Natural Science Disciplines
Genetic Structures
Molecular Structure
Disease
Disciplines and Occupations
Biochemical Phenomena
Chemical Phenomena
Genetic Phenomena
Tandem Repeat Sequences
Genetics
Laboratory Manuals
biology.
biological sciences.
physical sciences.
molecular structure.
disease.
illness.
Physical sciences
Molecular structure
Life sciences
Genomes
Diseases
Biology
Genetics
Nucleotides
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Hatters, Danny M
Hannan, Anthony J., 1969-
ISBN 9781627034388
1627034382
1627034374
9781627034371