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Book Cover

Title Trinucleotide repeat protocols / edited by Yoshinori Kohwi, Cynthia T. McMurray
Edition 2nd ed
Published New York : Humana Press, ©2013


Description 1 online resource (xiii, 296 pages) : illustrations
Series Methods in molecular biology, 1940-6029 ; v. 1010
Methods in molecular biology (Clifton, N.J.) ; volume 1010. 1064-3745
Contents A brief history of triplet repeat diseases -- Huntington's disease and cell therapies: Past, present, and future -- Transcranial two-photon imaging of synaptic structures in the cortex of awake head-restrained mice -- In vivo imaging of synapse plasticity in the mouse motor cortex -- Pluripotent hybrid stem cells from transgenic Huntington's disease monkey -- Mesenchymal stem cells for trinucleotide repeat disorders -- Lentiviral-mediated gene transfer of siRNAs for the treatment of Huntington's disease -- Purification of dFMR1-containing complexes using tandem affinity purification -- Combined FISH and immunofluorescent staining methods to co-localize proteins and mRNA in neurons and brain tissue -- Epigenetic modifications of the FMR1 gene -- The CGG repeat and the FMR1 gene -- Study of oxidative damage and antioxidant systems in two Huntington's disease rodent models -- Regulation of ataxin-1 phosphorylation and its impact on biology -- Towards understanding region-specificity of triplet repeat diseases: Coupled immunohistology and mass spectrometry imaging -- Antibodies and intrabodies against huntingtin: Production and screening of monoclonals and single-chain recombinant forms -- Cell recovery from DM1 transgenic mouse tissue to study (CTG) n instability and DM1 pathogenesis -- Markerless modification of trinucleotide repeat loci in BACs -- DRPLA: Recent advances in research using transgenic mouse models
Summary Two decades have passed since trinucleotide repeat expansion was first discovered in genes responsible for certain neurological diseases. Since then, new technologies have developed and innovative concepts have emerged, which may prove useful in devising therapeutic approaches to neurological diseases. Divided into six convenient sections, Trinucleotide Repeat Protocols, Second Edition covers a wide range of topics such as an overview of trinucleotide repeat diseases, synaptic plasticity, embryonic stem (ES) cell-related protocols with a focus on HD, RNA-related protocols, and analysis of epigenetic modification in fragile X syndrome. This edition focuses not only on direct analysis of trinucleotide repeat diseases but also on alternative approaches for the analysis of trinucleotide repeat diseases, with the hope that this will result in a better understanding of the mechanisms and future therapeutic prospects for treatment of these diseases. Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Trinucleotide Repeat Protocols, Second Edition seeks to serve researchers with its thorough methodologies on this expanding field
Analysis Medicine
Gene expression
Molecular Medicine
biomedische wetenschappen
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Subject Trinucleotide repeats -- Laboratory manuals
Genetic disorders.
Life sciences.
Nervous system -- Diseases.
Physical sciences.
Molecular structure.
Publication Formats
Microsatellite Repeats
Neurodegenerative Diseases
Genetic Diseases, Inborn
Tandem Repeat Sequences
Publication Characteristics
Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Biological Science Disciplines
Nervous System Diseases
Genome Components
Repetitive Sequences, Nucleic Acid
Natural Science Disciplines
Base Sequence
Disciplines and Occupations
Genetic Structures
Molecular Structure
Biochemical Phenomena
Genetic Phenomena
Phenomena and Processes
Chemical Phenomena
Laboratory Manuals
Heredodegenerative Disorders, Nervous System
Trinucleotide Repeats
biological sciences.
physical sciences.
molecular structure.
Physical sciences
Nervous system -- Diseases
Molecular structure
Life sciences
Genetic disorders
Trinucleotide repeats
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Kohwi, Yoshinori.
McMurray, Cynthia T
ISBN 9781627034111