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E-book

Title Genetic manipulation of secondary metabolites in medicinal plant / Ravi S. Singh, Nitish Kumar, editors
Published Singapore : Springer, [2023]

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Description 1 online resource
Series Interdisciplinary biotechnological advances
Interdisciplinary biotechnological advances.
Contents Intro -- Preface -- Acknowledgments -- Contents -- Chapter 1: Conventional Approaches Toward the Production of Bioactive Compounds from Medicinal Plants -- 1.1 Introduction -- 1.2 Medicinal Plants -- 1.3 Medicinal Plants Are Enriched with Bioactive Compounds -- 1.4 Major Areas of Bioactive Compounds -- 1.5 Production of Phytoceutical Compounds from Medicinal Plants -- 1.5.1 Increased Levels of Bioactive Compounds Through Different Farming Systems -- 1.5.2 Increased Levels of Bioactive Compounds Through Pruning Method
1.5.3 Increased Levels of Bioactive Compounds Through Traditional Breeding Methods -- 1.6 Conclusion -- References -- Chapter 2: RNA Interference: Novel Technique for Enhancing Secondary Metabolite Production in Medicinal Plants -- 2.1 Introduction -- 2.2 RNA Interference: History and Principle -- 2.3 Core Components of RNA Interference Machinery -- 2.3.1 Dicer -- 2.3.2 Argonaute (AGO) Proteins -- 2.3.3 RNA-Dependent RNA Polymerase (RdRP) -- 2.4 Mechanism of RNA Interference -- 2.4.1 Generation of siRNAs -- 2.4.2 Degradation of Target mRNA -- 2.5 RNA Interference in Plants
2.6 Application of RNA Interference in Enhancing Plant Secondary Metabolite Production in Plants -- 2.7 Advantages of RNA Interference -- 2.8 Limitations and Disadvantages of RNA Interference -- 2.9 Conclusion -- References -- Chapter 3: CRISPR/Cas9: A Novel Genetic Tool to Manipulate Plant Secondary Metabolite Pathways -- 3.1 Introduction -- 3.2 Applications of CRISPR in Medicinal Plants -- 3.2.1 Atropa belladonna -- 3.2.2 Cannabis sativa -- 3.2.3 Cichorium intybus L. -- 3.2.4 Dendrobium officinale -- 3.2.5 Dioscorea zingiberensis -- 3.2.6 Monochasma savatieri -- 3.2.7 Salvia miltiorrhiza
3.2.8 Rehmannia glutinosa -- 3.2.9 Symphytum officinale -- 3.2.10 Papaver somniferum L. -- 3.3 Limitations of CRISPR/Cas9 Technique -- 3.4 Future Perspectives -- References -- Chapter 4: Enhancement of Plant Secondary Metabolites by Genetic Manipulation -- 4.1 Introduction -- 4.2 Genetic Manipulation for SM -- 4.2.1 Mutagenesis and Recombination -- 4.2.2 Chemical and Physical Mutagenesis -- 4.2.3 Manipulation of Biosynthesis Genes -- 4.2.4 Elicitation with Biotic and Abiotic Elicitor -- 4.2.5 Transcription Factors -- 4.2.6 Manipulation of Biosynthesis Gene Cluster or Cryptic Gene
4.3 Cell Cultures as Biofactories -- 4.3.1 Metagenomics as a Source of Genetic Parts for SM Production in Bacteria -- 4.3.2 Heterologous Systems for SM Production -- 4.3.3 Plastid Manipulations -- 4.3.4 Glycosylation of Secondary Metabolites -- 4.3.5 Epigenetic Modifications -- 4.4 cDNA-AFLP as a Functional Genomics Tools -- 4.4.1 Modification of Translation Apparatus -- 4.4.2 Synthetic Biology -- 4.5 Conclusion and Future Perspective -- References -- Chapter 5: Role of Mutation and Stresses in the Production of Secondary Metabolite in Plants -- 5.1 Introduction -- 5.2 Plant Metabolites
Summary This book provides a comprehensive introduction and review of state-of-the-art biotechnological tools in enhancement of bioactive compounds in medicinal and aromatic plants. Plant bioactive compounds are plant-based natural products that display a variety of pharmacological applications. These bioactive compounds are important as medicines, pigments, flavorings and drugs because most of the pharmaceutical industries are highly dependent on medicinal plants and their extraction. The book introduces a systematic overview of state-of-the-art biotechnological techniques such as Omics, Crisper /Cas9 and RNAi to enhance the plant bioactive contents as well as enlists various in vitro techniques, hairy root culture and transgenic technology to enhance plant bioactive contents using plant tissue culture approaches. The book also provides an overview of the role of induced mutation, biotic and abiotic stress to increase the bioactive contents in plants and discusses the significant role of endophytes to enhance the contents of plant bioactive compound. The book also opens discussions related to standard operating procedures using hydroponics system of cultivation for significant enhancement of bioactive compound(s) and much more. This book serves as an excellent reference book for the researchers working in the field of plant secondary metabolites and pharmaceutical industries at global level
Notes Description based on online resource; title from digital title page (viewed on December 12, 2023)
Subject Plant metabolites -- Biotechnology
Medicinal plants -- Biotechnology
Medicinal plants -- Biotechnology.
Plant metabolites -- Biotechnology.
Form Electronic book
Author Singh, Ravi S., editor
Kumar, Nitish, editor.
ISBN 9789819949397
9819949394