Title Gene therapy of cancer : methods and protocols / edited by Wolfgang Walther

Edition Third edition Published New York, NY : Humana Press, [2022]

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Description 1 online resource (xi, 342 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; 2521 Springer protocols Springer protocols (Series) 1949-2448 Methods in molecular biology (Clifton, N.J.) ; v. 2521. 1064-3745 Contents Brief Introduction to Current Cancer Gene Therapy -- Minicircles for CAR T Cell Production by Sleeping Beauty Transposition -- A Technological Overview -- Generation of CAR-T Cells with Sleeping Beauty Transposon Gene Transfer -- Generation of Redirected Engineered Human Chimeric Antigen Receptor (CAR) T cells -- TCR- and CAR-engineering of Primary Human T cells -- TCR Gene Therapy for Cancer -- Gene Gun Her2/neu DNA Vaccination: Evaluation of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model -- Construction of Minicircle Suicide Genes Coding for Ribosome-Inactivating Proteins -- Claudin-targeted Suicide Gene Therapy for Claudin-Overexpressing Tumor Cells by Using Modified Clostridium Perfringens Enterotoxin (CPE) -- Bioengineered RNA Therapy in Patient-Derived Organoids and Xenograft Mouse Models -- Application of Transcription Factor Decoy Oligodeoxynucleotides (ODNs) for Cancer Therapy -- NK Cell Effector Functions and Bystander Tumor Cell Killing in Immunovirotherapy -- A qPCR-based Method for Quantification of Replication Competent Adenovirus (RCA) in Conditionally Replicating Oncolytic Adenoviruses -- Visualized Cancer Immunotherapy with Engineered Salmonella typhimurium -- CANCER Gene Therapy: Development and Production Of Lentiviral Vectors For Gene Therapy -- Capillary Gel Electrophoresis (CGE) for Quality Control of Plasmid DNA in Gene Therapy: quality control of 20-years stored GMP-grade Plasmid DNA -- Efficient Generation of Superior Dumbbell-Shaped Non-viral DNA Delivery Vectors Using 1-2-3 Gap-primer PCR Summary This third edition provides new and updated chapters on gene therapeutic strategies of cancer. Chapters guide readers through suicide and oncolytic gene therapy, gene replacement and gene suppression therapy, vector development and refinement, immunogene therapy, TCR and CAR engineering, tumor vaccination using DNA or RNA vaccines, and antitumoral immune stimulation at different levels. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Gene Therapy of Cancer: Methods and Protocols, Third Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed March 17, 2023) Subject Cancer -- Gene therapy -- Laboratory manuals Genetic Therapy -- methods Neoplasms -- therapy Cancer -- Gene therapy Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Walther, Wolfgang, editor ISBN 9781071624418 1071624415

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