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Book Cover
E-book
Author Hochstrat, Rita, author

Title Immobilized biocatalysts for bioremediation of groundwater and wastewater / Rita Hochstrat, Thomas Wintgens and Philippe Corvini ; contributors, Spiros N. Agathos [and thirty others]
Published London, England : Iwa Publishing, 2015
©2015

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Description 1 online resource (178 pages) : illustrations (some color), photographs, tables
Series European Water Research Series
EU report series.
Contents Cover; Copyright; Contents; List of figures; List of tables; List of contributors; Preface; Acknowledgement; Abbreviations; Chapter 1: Introduction; 1.1 Pollutants in the Aquatic Environment; 1.1.1 Types, occurrence and fate; 1.1.2 Regulatory frameworks; 1.1.2.1 EU level legislation; 1.1.2.2 National legislation; 1.2 Environmental Biotechnology Options; 1.2.1 Challenges for the implementation of bioaugmentation; 1.2.2 Challenges for the use of enzymes; 1.2.3 Immobilization of whole cells and enzymes as a solution of choice to circumvent bioremediation difficulties
1.3 Recent Research -- The MINOTAURUS Project1.3.1 Scope and ambition; 1.3.2 Approach; 1.4 About this Book; 1.5 References; Chapter 2: Analytical and monitoring methods; 2.1 Chemical Methods for the Analysis of Target Pollutants; 2.1.1 Endocrine disrupting compounds and pharmaceuticals; 2.1.1.1 Liquid chromatography for the analysis of polar pharmaceuticals; 2.1.1.2 Analysis of BPA with GC-MS; 2.1.1.3 Simulateneous measurement of BPA, CBZ, DF, EE2, NP, SMX, and TCS; 2.1.2 Methyl tert-butyl ether, tert-butyl alcohol and chlorinated aliphatic hydrocarbons; 2.2 Isotopic Methods
2.2.1 14C-Radioanalytics2.2.1.1 Background and potential; 2.2.1.2 Application in the MINOTAURUS project; 2.2.2 Compound specific stable isotope analysis (CSIA); 2.2.2.1 Exemplified CSIA application in a technical processes; 2.3 Biocatalyst Monitoring; 2.3.1 Monitoring tools for microorganisms; 2.3.1.1 Fluorescence in situ hybridization (FISH); 2.3.1.2 Quantitative polymerases chain reaction (qPCR); 2.3.1.3 Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE); 2.3.1.4 Next generation sequencing; 2.3.1.5 Stable isotope probing (SIP); 2.3.1.6 BACTRAPs
2.3.2 Monitoring tools for enzymes2.3.2.1 Colorimetric assays for measuring laccase activity; 2.3.2.2 Determining oxygen consumption rate (OCR); 2.3.2.3 Determining enzyme activity via co-factor oxidation in the UV-range; 2.3.2.4 Assessment of enzyme kinetics with target pollutants using chemical analysis; 2.4 Ecotoxicity Monitoring; 2.4.1 Batteries of ecotoxicity tests; 2.4.2 Ecotoxicity tests used within the MINOTAURUS project; 2.5 References; Chapter 3: Immobilization techniques for biocatalysts; 3.1 Introduction; 3.2 Immobilization of Biomass; 3.2.1 Bioaugmented membrane bioreactor (MBR)
3.2.1.1 Immobilization on carrier material3.2.1.2 Encapsulation in alginate beads; 3.2.2 Bioaugmented packed bed reactor (PBR); 3.2.2.1 Biomass immobilization for the development of packed bed reactors treating chlorinated aliphatic hydrocarbons (CAH); 3.2.3 Microorganisms on electrically conductive carriers; 3.2.3.1 Tailored electrically-conductive carrier materials; 3.3 Immobilization of enzymes; 3.3.1 Bio-inspired titanification; 3.3.1.1 Principle; 3.3.1.2 Biocatalyst performance; 3.3.2 Enzyme conjugated nanoparticles; 3.3.2.1 Principle; 3.3.2.2 Biocatalyst performance; 3.4 References
Summary The European project MINOTAURUS explored innovative bio-processes to eliminate emerging and classic organic pollutants. These bio-processes are all based on the concept of immobilization of biocatalysts (microorganisms and enzymes) and encompass bioaugmentation, enzyme technology, rhizoremediation with halophytes, and a bioelectrochemical remediation process. The immobilization-based technologies are applied as engineered ex situ treatment systems as well as natural systems in situ for the bioremediation of groundwater, wastewater and soil. The selection and application of tailored physico-chemical, molecularbiological and ecotoxicological monitoring tools combined with a rational understanding of engineering, enzymology and microbial physiology is a pertinent approach to open the black-box of the selected technologies. Reliable process monitoring constitutes the basis for developing and refining biodegradation kinetics models, which in turn improve the predictability of performances to be achieved with technologies. Immobilised Biocatalysts for Bioremediation of Groundwater and Wastewater delivers insight into the concepts and performance of a series of remediation approaches. A key strength of this book is to deliver results from lab-scale through to piloting at different European reference sites. It further suggests frameworks for structuring and making evidence-based decisions for the most appropriate bioremediation measures
Bibliography Includes bibliographical references
Notes Online resource; title from PDF title page (ebrary, viewed April 10, 2015)
Subject Sewage -- Purification -- Biological treatment.
Land treatment of wastewater.
Land treatment of wastewater
Sewage -- Purification -- Biological treatment
Form Electronic book
Author Wintgens, Thomas, author
Corvini, Philippe, author
Agathos, Spiros N., contributor
ISBN 9781780406466
1780406460
9781523102556
1523102551