| Description |
1 online resource (270 pages) |
| Contents |
Principles and Applications of Fluorescence Spectroscopy; Contents; 1 Absorption Spectroscopy Theory; 2 Determination of the CalcofluorWhite Molar Extinction Coefficient Value in the Absence and Presence of a1-Acid Glycoprotein; 3 Determination of Kinetic Parameters of Lactate Dehydrogenase; 4 Hydrolysis of p-Nitrophenyl-B-D-Galactoside with B-Galactosidase from E. coli; 5 Starch Hydrolysis by Amylase; 6 Determination of the pK of a Dye; 7 Fluorescence Spectroscopy Principles; 8 Effect of the Structure and the Environment of a Fluorophore on Its Absorption and Fluorescence Spectra |
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9 Fluorophore Characterization and Importance in Biology10 Fluorescence Quenching; 11 Fluorescence Polarization; 12 Interaction Between Ethidium Bromide and DNA; 13 Lens culinaris Agglutinin: Dynamics and Binding Studies; 14 Förster Energy Transfer; 15 Binding of TNS on Bovine Serum Albumin at pH 3 and pH 7; 16 Comet Test for Environmental Genotoxicity Evaluation: A Fluorescence Microscopy Application; 17 Questions and Exe |
| Summary |
Fluorescence spectroscopy is an important investigational tool in many areas of analytical science, due to its extremely high sensitivity and selectivity. With many uses across a broad range of chemical, biochemical and medical research, it has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems with extremely high resolution. It is particularly heavily used in the pharmaceutical industry where it has almost completely replaced radiochemical labelling. Principles and Applications of Fluorescence Spect |
| Notes |
Print version record |
| Subject |
Biochemistry.
|
|
Fluorescence spectroscopy.
|
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Biochemistry
|
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Spectrometry, Fluorescence
|
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biochemistry.
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Biochemistry
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Fluorescence spectroscopy
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| Form |
Electronic book
|
| ISBN |
9780470691335 |
|
0470691336 |
|
1281319007 |
|
9781281319005 |
|
