| Description |
1 online resource (x, 246 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 153 |
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Methods in molecular biology (Clifton, N.J.) ; v. 153
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| Contents |
pt. 1. Cloning techniques -- Cloning neuropeptide tyrosine cDNA -- Human Y1/Y5 receptor gene cluster: isolation and characterization -- Human type 2 neuropeptide Y receptor gene: isolation and characterization -- Y4 receptor in different species: functional expression and binding -- Neuropeptide Y Y5 receptor expression -- Homology-based cloning methods: identification of the NPY Y2, Y4, and Y6 receptors -- pt. 2. Production of transgenic and knock-out models -- Developing transgenic neuropeptide Y rats -- Neuropeptide Y Y1 receptor-deficient mice: generation and characterization -- pt. 3. Antisense technology -- Antisense oligonucleotide apporach to study NPY-mediated feeding signal transduction -- A G[alpha]i RNA-antisense experssion strategy to investigate coupling of peptide YY/neuropeptide Y receptor to G[alpha]i -- NPY Y5 receptor subtype: pharmacological characterization with antisense oligodeosynucleotide screening strategy -- NPY antisense oligodeoxynucleotides to study the actions of NPY -- pt. 4. Techniques to study tissue distribution of mRNA -- Localization of Y-receptor subtype mRNAs in rat brain by digoxigenin labeled in situ hybridization -- Central Y4 receptor distribution: radioactive ribonucleotide probe in situ hybridization in vitro receptor autoradiography -- Combining non-isotopic localization of NPY mRNA with immunocytochemistry -- pt. 5. Miscellaneous techniques -- Y receptors characterized by RT-PCR: distribution in rat intestine -- Quantification of NPY mRNA by ribonuclease protection assay -- Radioligand binding studies: pharmacological profiles of cloned Y-receptor subtypes |
| Summary |
It is now clear that neuropeptide Y (NPY), the most abundant peptide present in the mammalian nervous system, is implicated in the pathophysiology of many diseases, including eating disorders, seizures, memory loss, anxiety, depression, and cardiovascular diseases, and offers major therapeutic opportunities for their treatment. In Neuropeptide Y Protocols, a strong cohort of established investigators from a variety of disciplines detail their best techniques for advancing NPY research. Among the many readily reproducible methods described are those for cloning, for the production of transgenic and knockout models, and for the use of antisense technology to define the receptors and the signal transduction pathways mediating NPY actions. A range of the major qualitative and quantitative techniques used to study tissue mRNA distribution are also included, as well as methods for radioligand binding. The cloning techniques utilize genomic DNA isolation, expression cloning, PCR cloning, the construction of hybrid receptors, and homology-based cloning. Highly practical and state-of-the-art, Neuropeptide Y Protocols constitutes a gold-standard collection of proven techniques for advancing today's understanding of this significant neuropeptide, as well as for developing the promising NPY-based therapeutics now emerging for many important disorders |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| In |
Springer Protocols |
| Subject |
Neuropeptide Y -- Laboratory manuals
|
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Neuropeptide Y.
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Neuropeptide Y
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SOCIAL SCIENCE -- Anthropology -- Physical.
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Neuropeptide Y
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Wetenschappelijke technieken.
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| Genre/Form |
Handbooks and manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Balasubramaniam, Ambikaipakan.
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| ISBN |
0896036626 |
|
9780896036628 |
|
9781592590421 |
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159259042X |
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1280821639 |
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9781280821639 |
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9786610821631 |
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6610821631 |
|
