| Description |
1 online resource : illustrations |
| Contents |
Front Cover; STEM CELL MANUFACTURING; STEM CELL MANUFACTURING; Copyright; CONTENTS; LIST OF CONTRIBUTORS; INTRODUCTION; 1 -- Genetic Engineering in Stem Cell Biomanufacturing ; 1.1 INTRODUCTION; 1.2 GENETIC MANIPULATION APPROACHES IN HUMAN PLURIPOTENT STEM CELLS; 1.2.1 Transgenic Approaches; 1.2.2 Knock-In and Knock-Out Approaches; 1.2.2.1 Zinc-Finger Nucleases; 1.2.2.2 Transcription Activator-Like Effector Nucleases; 1.2.2.3 Clustered Regularly Interspaced Short Palindromic Repeat/Cas9; 1.2.3 Bacterial Artificial Chromosomes; 1.3 APPLICATIONS |
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1.3.1 Genetic Labeling for Cell Identification and Cell Tracking1.3.2 Gene Alteration for Directed Differentiation; 1.3.3 Gene Disruption for Functional Investigation; 1.3.4 Gene Correction for Function Restoration; 1.4 DELIVERY METHODS; 1.4.1 Transfection; 1.4.2 Nucleofection; 1.4.3 Viral Transduction; 1.5 CONCLUSIONS; ACKNOWLEDGMENTS; REFERENCES; 2 -- Biomechanics in Stem Cell Manufacturing; 2.1 INTRODUCTION; 2.2 CELLULAR BIOMECHANICS; 2.2.1 Biomechanical Cues; 2.2.2 Shear Forces and Differentiated Cells; 2.2.3 Shear Forces and Pluripotent Stem Cells |
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2.3 SCALE UP TOWARD PRODUCTION-LEVEL BIOREACTORS2.4 BIOMANUFACTURING CELLS FOR THERAPIES; 2.4.1 Pluripotent Stem Cells; 2.4.2 Cardiomyocytes; 2.4.3 Endothelial Cells; 2.5 CONCLUSION; REFERENCES; 3 -- Bioreactor Engineering Fundamentals for Stem Cell Manufacturing ; 3.1 INTRODUCTION; 3.2 STIRRED BIOREACTOR BASICS; 3.3 SPECIAL FEATURES OF STIRRED BIOREACTORS FOR HMSC CULTURE ON MICROCARRIERS; 3.3.1 Introduction; 3.3.2 Preparing the Bioreactor for Culture; 3.3.3 Medium and Medium Exchange; 3.3.4 Microcarrier Selection; 3.3.5 Cell and Microcarrier Concentrations; 3.3.6 Attachment Protocol |
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3.4.1 Increasing Cell Density3.4.2 Oxygen Demand and Mass Transfer at Higher Cell Density-Sparging and Higher Agitator Speeds; 3.4.3 Carbon Dioxide, Osmolality, and pH; 3.4.4 Human-Induced and Embryonic Pluripotent Stem Cells; 3.5 CONCLUSIONS; NOMENCLATURE; REFERENCES; 4 -- Microcarrier Culture Systems for Stem Cell Manufacturing ; 4.1 OVERVIEW; 4.1.1 Historical Perspective; 4.2 MICROCARRIER TECHNOLOGY; 4.2.1 Types of Microcarriers; 4.2.2 Properties of Microcarriers Required for Cell Culturing; 4.2.3 Advantages of Using Microcarrier Culture Systems for Cell Manufacturing |
| Notes |
<P>1. Genetic Engineering in Stem Cell Manufacturing<i></p></i> <p>2. Biomechanics in Stem Cell Manufacturing<i></p></i> <p>3. Bioreactor Engineering Fundamentals for Stem Cell Manufacturing<i></p></i> <p>4. Microcarrier Culture Systems for Stem Cell Manufacturing<i></p></i> <p>5. Novel Single-Use Bioreactors for Scale up of Anchorage-Dependent Cell Manufacturing for Cell Therapies<i></p></i> <p>6. Bioreactors and Downstream Processing for Stem Cell Manufacturing<i></p></i> <p>7. Cell production system based on flexible modular platform<i></p></i> <p>8. Microfluidic Devices for the Culture of Stem Cells</p><i> <p></i>9. Enrichment and Separation Technologies for Stem Cell-Based Therapies<i> </p></i> <p>10. Expansion and Characterization Considerations for the Manufacturing of Stem Cells</p><i> <p></i>11. Storage and Delivery of Stem Cells for Cellular Therapies<i></p></i> <p>12. Business Models for Manufacture of Cellular Therapies<i></p></i> <p>13. Stem cells for the regeneration of chronic wounds: a translational perspective<i></p></i> |
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CIP data; resource not viewed |
| Subject |
Bioengineering.
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Stem cells -- Therapeutic use
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Stem cells -- Transplantation.
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Biotechnology.
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bioengineering.
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Biotechnology
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Bioengineering
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Stem cells -- Transplantation
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| Form |
Electronic book
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| Author |
Silva, Claudia Lobato da, author
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Diogo, Maria Fonseca Rodrigues, author
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Chase, Lucas G., author
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| ISBN |
9780444632661 |
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0444632662 |
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