| Description |
1 online resource (xiv, 458 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 415 |
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Methods in molecular biology (Clifton, N.J.) ; v. 415
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| Contents |
ENU mutagenesis in mice / Philippe Georgel [and others] -- Innate immunity genes as candidate genes : searching for relevant natural polymorphisms in databases and assessing family-based association of polymorphisms with human diseases / Pascal Rihet -- KIR locus polymorphisms : genotyping and disease association analysis / Maureen P. Martin and Mary Carrington -- Experimental model for the study of the human immune system : production and monitoring of "human immune system" Rag2-/[gamma]c-/- mice / Nicolas Legrand, Kees Weijer, and Hergen Spits -- Bacterial artificial chromosome transgenesis through pronuclear injection of fertilized mouse oocytes / Kristina Vintersten [and others] -- Bioluminescence imaging to evaluate infections and host response in vivo / Pamela Reilly Contag -- Intravital two-photon imaging of natural killer cells and dendritic cells in lymph nodes / Susanna Celli, Béatrice Breart, and Philippe Bousso -- Dissection of the antiviral NK cell response by MCMV mutants / Stipan Jonjic [and others] -- Analyzing antibody-Fc-receptor interactions / Falk Nimmerjahn and Jeffrey V. Ravetch |
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The isolation and analysis of human natural killer cell subsets / Guido Ferlazzo -- Innate immune function of eosinophils : from antiparasite to antitumor cells / Fanny Legrand [and others] -- Ex vivo and in vitro primary mast cells / Michel Arock [and others] -- Murine macrophages : a technical approach / Luisa Martinez-Pomares and Siamon Gordon -- Clinical analysis of dendritic cell subsets : the dendritogram / Anne Hosmalin, Miriam Lichtner, and Stéphanie Louis -- Clinical analysis of human natural killer cells / Pascale André and Nicolas Anfossi -- Lentiviral transduction of immune cells / Louise Swainson [and others] -- Axenic mouse model / Antoine Giraud -- In vivo analysis of zebrafish innate immunity / Jean-Pierre Levraud [and others] -- Reverse genetics analysis of antiparasitic responses in the malaria vector, Anopheles gambiae / Stephanie A. Blandin and Elena A. Levashina -- Drosophila immunity : methods for monitoring the activity of toll and Imd signaling pathways / Yves Romeo and Bruno Lemaitre -- Investigating the involvement of host factors involved in intracellular pathogen infection by RNAi in Drosophila cells / Hervé Agaisse -- Models of Caenorhabditis elegans infection by bacterial and fungal pathogens / Jennifer R. Powell and Frederick M. Ausubel -- Genetic analysis of Caenorhabditis elegans innate immunity / Michael Shapira and Man-Wah Tan -- Measuring cell-wall-based defenses and their effect on bacterial growth in Arabidopsis / Min Gab Kim and David Mackey |
| Summary |
Immunologists today are interested in all of the diverse cell-types involved in host defense and have a deeper appreciation of the importance of innate immune mechanisms as a first line of protection against pathogens. This volume thus discusses the isolation and functional characterization of cells involved in innate immunity in mouse and man, including mast cells and eosinophils. Other focuses include natural killer cells, methods in statistics, in vivo imaging, genome engineering, and mutagenesis and culture that are adapted to the study of innate immunity in these hosts. These are complemented with a series of chapters dealing with alternative models: plants, worms, mosquitoes, flies, and fish. Together, these approaches and models are being used to dissect the complex interplay between hosts and pathogens and contribute to developing strategies to help fight infection. With chapters written by experts on the cutting-edge of this technology, Innate Immunity is an essential reference for immunologists, histologists, geneticists, and molecular biologists |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Natural immunity.
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Immunology, Comparative.
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Killer cells.
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Immunity, Innate
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Immunologic Techniques
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Killer Cells, Natural
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Killer cells.
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Immunology, Comparative.
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Natural immunity.
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| Genre/Form |
Laboratory manuals.
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Ewbank, Jonathan
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Vivier, E
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| ISBN |
9781597455701 |
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1597455709 |
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