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Title CRISPR guide RNA design : methods and protocols / edited by Tudor A. Fulga, David J.H.F. Knapp, and Quentin R.V. Ferry
Published New York, NY : Humana Press, [2021]

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Description 1 online resource (1 PDF file (x, 286 pages)) : illustrations
Series Methods in molecular biology, 1940-6029 ; volume 2162
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 2162. 1064-3745
Springer protocols (Series) 1949-2448
Summary This volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls
Bibliography Includes bibliographical references and index
Notes Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL
Online resource; title from PDF title page (SpringerLink, viewed November 11, 2020)
Subject RNA editing -- Laboratory manuals
CRISPR (Genetics) -- Laboratory manuals
Clustered Regularly Interspaced Short Palindromic Repeats
RNA Editing
Biomedical engineering.
Genetics (non-medical).
Technology & Engineering -- Biomedical.
Science -- Life Sciences -- Genetics & Genomics.
CRISPR (Genetics)
RNA editing.
Genre/Form Laboratory manuals.
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Fulga, Tudor A., editor
Knapp, David J. H. F., editor
Ferry, Quentin R. V., editor
ISBN 9781071606872
1071606875