| Description |
1 online resource (xvii, 256 pages) : illustrations |
| Series |
Methods in molecular biology ; 397 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 397
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| Contents |
Purifying the hedgehog protein and its variants / Darren P. Baker, Frederick R. Taylor, and R. Blake Pepinsky -- Application of sonic hedgehog to the developing chick limb / Eva Tieck and Cheryll Tickle -- Manipulation of hedgehog signaling in Xenopus by means of embryo microinjection and application of chemical inhibitors / Thomas Hollemann . [and others] -- Isolation of rat telencephalic neural explants to assay Shh GABAergic interneuron differentiation-inducing activity / Rina Mady and Jhumku D. Kohtz -- Genetic analysis of the vertebrate hedgehog-signaling pathway using muscle cell fate specification in the zebrafish embryo / Sudipto Roy -- Efficient manipulation of hedgehog/GLI signaling using retroviral expression systems / Maria Kasper [and others] -- Cell surface marker and cell cycle analysis, hedgehog signaling, and flow cytometry / Krsting Detmer and Ronald E. Garner -- Detecting tagged hedgehog with intracellular and extracellular immunocytochemistry for functional analysis / Ainhoa Callejo, Luis Quijada, and Isabel Guerrero -- Confocal analysis of hedgehog morphogenetic gradient coupled with fluorescent in situ hybridization of hedgehog target genes / Armel Gallet and Pascal P. Thérond -- RNA in the hedgehog signaling pathway : pFRiPE, a vector for temporally and spatially controlled RNAi in Drosophila / Eric Marois and Suzanne Eaton -- Germline clone analysis for maternally acting Drosophila hedgehog components / Erica M. Selva and Beth E. Stronach -- Clone analysis of hedgehog signaling in Drosophila somatic tissues / Christing M. Bankers and Joan E. Hooper -- GAL4/UAS targeted gene expression for studying Drosophila hedgehog signaling / Denise Bussona nd Anne-Marie Pret -- Biochemical fractionation of Drosophila cells / Melanie Stegman and David Robbins -- Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway / Chao Ton and Jin Jiang -- Sequence analyses to study the evolutionary history and cis-regulatory elements of hedgehog genes / Ferenc Müller and Anne-Gaelle Borycki |
| Summary |
In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the€pathway, which makes€it an incredibly important system to understand. This volume€examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible€and comprehensive text.€This€volume provides a single source€for investigators, presenting€several different experimental€approaches, which are all varied and broad in nature, as well as procedures for a€variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Cellular signal transduction -- Research -- Methodology
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Transcription factors -- Research -- Methodology
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Genetic regulation.
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Cellular signal transduction.
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Gene Expression Regulation
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Hedgehog Proteins
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Microbiological Techniques
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Signal Transduction
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Transcription factors -- Research -- Methodology.
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Cellular signal transduction -- Research -- Methodology.
|
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Genetic regulation
|
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Cellular signal transduction
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Cellular signal transduction -- Research -- Methodology
|
| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Horabin, Jamila I
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| ISBN |
9781597455169 |
|
1597455164 |
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1603271236 |
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9781603271233 |
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1280971827 |
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9781280971822 |
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9786610971824 |
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661097182X |
|
