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Author Lesnik, Chen

Title Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description 1 online resource (streaming video file) (884 secs.)
Series Biology
Summary Many mRNAs encoding mitochondrial proteins are associated with the mitochondria outer membrane. We describe a subcellular fractionation procedure aimed at isolation of yeast mitochondria with its associated mRNAs and ribosomes. This protocol can be applied to cells grown under diverse conditions in order to reveal mechanisms of mRNA localization and localized translation near the mitochondria
Audience For undergraduate, graduate, and professional students
Notes English
Description based on online resource; title from resource description page
Subject Biology
Form Streaming audio