Book Cover
E-book

Title TGF-Beta signaling : methods and protocols / edited by Xin-Hua Feng, Pinglong Xu, Xia Lin
Published New York : Humana Press : Springer, 2016
©2016

Copies

Description 1 online resource (xii, 394 pages) : illustrations (some color)
Series Methods in molecular medicine ; 1344
Methods in molecular biology (Clifton, N.J.) ; 1344
Contents Regulation of TGF-beta receptors / Erine H. Budi, Jian Xu, and Rik Derynck -- 2 Determining TGF-beta receptor levels in the cell membrane / Long Zhang [and others] -- Posttranslational modifications of TGF-beta receptors / Xiaohua Yan and Ye-Guang Chen -- Production, isolation, and structural analysis of ligands and receptors of the TGF-beta superfamily / Tao Huang and Andrew P. Hinck -- Phosphorylation of Smads by intracellular kinases / Fang Liu and Isao Matsuura -- Analysis of Smad phosphatase activity in vitro / Tao Shen, Lan Qin, and Xia Lin -- Three-dimensional mammary epithelial cell morphogenesis model for analysis of TGFbeta signaling / Juliet Rashidian and Kunxin Luo -- TGF-beta signaling in stem cell regulation / Wenlin Li, Wanguo Wei, and Sheng Ding -- Analysis of epithelial-mesenchymal transition induced by transforming growth factor beta / Ulrich Valcourt [and others] -- In vitro Th differentiation protocol / Takashi Sekiya and Akihiko Yoshimura -- Interrogating TGF-beta function and regulation in endothelial cells / J.A. Maring [and others] -- Isolation and manipulation of adipogenic cells to assess TGF-beta superfamily functions / Maria Namwanje, Juan C. Bournat, and Chester W. Brown -- Imaging TGF-beta signaling in mouse models of cancer metastasis / Yibin Kang -- Generation and characterization of Smad7 conditional knockout mice / Yi Pan and Yan Chen -- Monitoring Smad activity in vivo using the Xenopus model system / Marco Montagner, Graziano Martello, and Stefano Piccolo -- Animal cap assay for TGF-beta signaling / Chenbei Chang -- Detection of Smad signaling in Zebrafish embryos / Xingfeng Liu, Qiang Wang, and Anming Meng -- Role of TGF-beta signaling in coupling bone remodeling / Janet L. Crane, Lingling Xian, and Xu Cao -- Studying the functions of TGF-beta signaling in the ovary / Chao Yu, Jian-Jie Zhou, and Heng-Yu Fan -- Quantitative real-time PCR analysis of MicroRNAs and their precursors regulated by TGF-beta signaling / Hara Kang and Akiko Hata -- TGF-beta-regulated MicroRNAs and their function in cancer biology / Pengyuan Yang [and others] -- Epigenomic regulation of Smad1 signaling during cellular senescence induced by Ras activation / Atsushi Kaneda [and others] -- Role of ubiquitination to Determine non-Smad signaling responses / Shyam Kumar Gudey and Marene Landström -- Genome-wide RNAi screening to dissect the TGF-beta signal transduction pathway / Xiaochu Chen and Lan Xu -- Measuring TGF-beta ligand dynamics in culture medium / Zipei Feng, Zhike Zi, and Xuedong Liu
Bibliography Includes bibliographical references and index
Subject Transforming growth factors-beta -- Receptors -- Laboratory manuals
Cellular signal transduction.
Proteins.
Receptors, Transforming Growth Factor beta
Signal Transduction
Proteins
protein.
Cellular biology (cytology)
Science -- Life Sciences -- Biology -- Molecular Biology.
Cellular signal transduction
Proteins
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Feng, Xin-Hua, editor.
Xu, Pinglong, editor.
Lin, Xia (Professor of Surgery), editor.
LC no. 2015951954
ISBN 9781493929665
1493929666
1493929658
9781493929658