| Description |
1 online resource : illustrations (some color) |
| Contents |
1 -- INTRODUCTION TO VIRAL STRUCTURE, DIVERSITY AND BIOLOGY ; 1.2 OVERVIEW OF VIRAL STRUCTURE: CAPSIDS, GENOMES, AND ENVELOPES ; 1.2.1 Capsids ; 1.2.2 Genomes ; 1.2.3 Envelopes ; 1.2.4 Additional Virion Components ; 1.3 VIRUS DIVERSITY AND CLASSIFICATION: ORIGIN OF VIRUSES ; 1.4 APPROACHES TO VISUALIZE VIRUSES ; 1.5 VIRAL LIFE CYCLES: LYTIC VERSUS LATENT ; 1.5.1 Adsorption/Attachment |
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1.5.2 Penetration/Viral Entry 1.5.3 Biosynthesis ; 1.5.4 Assembly ; 1.5.5 Release ; Further Reading ; 2 -- Viral Tools for In Vitro Manipulations of Nucleic Acids: Molecular Cloning ; 2.1 Overview of Molecular Cloning ; 2.1.1 Structure and Chemical Composition of Nucleic Acids: Flow of Genetic Information ; 2.1.2 Principles of Molecular Cloning ; 2.2 Vectors and Other Nonenzyme Tools Derived From Viruses ; 2.2.1 Viral Vectors ; 2.2.2 Virus-Based DNA Ladders ; 2.2.3 Phage-Resistant Escherichia coli Strains |
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2.3 Virus-Related Enzymes in Recombinant DNA Technology 2.3.1 Restriction Enzymes and Viral Nucleases ; 2.3.1.1 Restriction Enzymes ; 2.3.1.2 Restriction Enzyme Applications ; 2.3.1.3 Sequence-Nonspecific DNA Nucleases ; 2.3.2 Replication-Related Enzymes ; 2.3.2.1 DNA Polymerases ; 2.3.2.2 Reverse Transcriptases ; 2.3.2.3 DNA Ligases ; 2.3.2.4 Topoisomerases ; 2.3.2.5 Single-Stranded DNA Proteins ; 2.3.3 DNA-Modifying Enzymes ; 2.3.3.1 Polynucleotide Kinase ; 2.3.3.2 Methyltransferases ; 2.4 Viral Tools for Manipulating RNA In Vitro |
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2.4.1 RNA Polymerases 2.4.2 Vaccinia Virus RNA Capping System ; 2.4.3 RNA Ligases ; Further Reading ; 3 -- Viral Tools for Genome Manipulations In Vivo ; 3.1 CONVENTIONAL APPROACHES TO GENOME MANIPULATIONS ; 3.2 VIRUS-BASED RECOMBINATION SYSTEMS AND THEIR APPLICATIONS ; 3.2.1 Virus-Driven Recombination Overview ; 3.2.2 Lambda Red Recombineering ; 3.2.3 Cre/LoxP Recombination System ; 3.2.4 Phage Integrases ; 3.3 GENOME EDITING ; 3.3.1 Genome Editing Overview ; 3.3.1.1 Meganucleases ; 3.3.1.2 Zinc-Finger Nucleases |
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3.3.1.3 Transcription Activatora-Like Endonucleases 3.3.1.4 The CRISPR/Cas9 system ; 3.3.2 Key Applications of Nuclease-Based Editing ; 3.3.3 Biology of the CRISPR/Cas-Based Adaptive Immune System in Prokaryotes: The Big Picture ; 3.3.3.1 CRISPR-Based Adaptive Immunity: Components and Mechanism ; 3.4 RNA INTERFERENCE ; Further Reading ; 4 -- Viral Tools for Protein Expression and Purification ; 4.1 Overview of Protein Expression: Challenges and Limitations ; 4.2 Viral Components Utilized in Protein Expression Systems |
| Summary |
Harnessing the Power of Viruses explores the application of scientific knowledge about viruses and their lives to solve practical challenges and further advance molecular sciences, medicine and agriculture. The book contains virus-based tools and approaches in the fields of: i) DNA manipulations in vitro and in vivo; ii) Protein expression and characterization; and iii) Virus- Host interactions as a platform for therapy and biocontrol are discussed. It steers away from traditional views of viruses and technology, focusing instead on viral molecules and molecular processes that enable science to better understand life and offer means for addressing complex biological phenomena that positively influence everyday life. The book is written at an intermediate level and is accessible to novices who are willing to acquire a basic level of understanding of key principles in molecular biology, but is also ideal for advanced readers interested in expanding their biological knowledge to include practical applications of molecular tools derived from viruses |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (EBSCO, viewed November 15, 2017) |
| Subject |
Viruses.
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Virology.
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SCIENCE -- Life Sciences -- Biology.
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SCIENCE -- Life Sciences -- Microbiology.
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Virology
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Viruses
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| Form |
Electronic book
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| ISBN |
9780128105153 |
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0128105151 |
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