| Description |
xii, 212 pages : illustrations (some color) ; 25 cm |
| Series |
Oxford medical publications |
|
Oxford medical publications.
|
| Contents |
1. Principles of in situ hybridization / Heinz Hofler, James Mueller and Martin Werner -- 2. The design and generation of probes for in situ hybridization / Mary E. Sunday -- 3. The preparation of non-radioisotopic hybridization probes / Christopher J. Bakkenist and James O'D. McGee -- 4. Principles and applications of complementary RNA probes / J. H. Steel, L. Gordon and J. M. Polak -- 5. Quantification of in situ hybridization macro-autoradiograms / Anthony P. Davenport -- 6. Quantification of grains in in situ hybridization / Jane Aldridge -- 7. Detection of genetic changes in cancer by interphase cytogenetics and comparative genomic hybridization / Anton H. N. Hopman, Christina E. M. Voorter and Ernst J. M. Speel [et al.] -- 8. Single and simultaneous nucleic acid detection in clinical samples / C. S. Herrington and James O'D. McGee |
|
9. Combined in situ hybridization and immunocytochemistry; investigation of viral pathogenesis at the light and electron microscope levels / Kenneth A. Fleming and Adrienne L. Morey -- 10. Supersensitive in situ DNA and RNA localization / Gerhard W. Hacker, Ingebord Zehbe and Raymond Tubbs |
| Summary |
This practical guide covers all aspects of in situ hybridization, describing, practical procedures and protocols, the scientific background, areas of application, and the limitations of technology. This new edition has been completely rewritten by the experts in the field to take into account the many advances (including PCR in situ) in the last seven years. Each chapter contains up-to-date references, the latest advances, and prospects for the future |
| Notes |
Previous ed.: 1990 |
| Bibliography |
Includes bibliographies |
| Subject |
In situ hybridization.
|
|
Molecular biology -- Technique.
|
|
Nucleic acid hybridization.
|
| Author |
McGee, J. O'D. (James O'Donnell)
|
|
Polak, Julia M.
|
| LC no. |
98039953 |
| ISBN |
019854880X (hc.) |
|
