| Description |
1 online resource (xviii, 616 pages) : illustrations |
| Series |
Methods in molecular medicine ; 9 |
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Methods in molecular medicine ; 9.
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| Contents |
Light microscopy : Overview and basic methods / A.J. Leathem and S.A. Brooks-Lectin histochemistry and cytochemistry-light microscopy : Avidin-biotin amplification on resin-embedded sections / R.W. Stoddart and C.J. Jones-Lectin-gold histochemistry on paraffin and lowicryl k4m sections using biotin and digoxigenin-conjugated lectins / J. Roth [and others]-Use of fluorochrome-labeled lectins in light microscopy / U. Schumacher and B.S. Mitchell-The use of lectins in combination with enzymatic digestion for the study of glycoconjugates in cartilage / S. Hoebt-Schmidt-Applications of lectin histochemistry and cytochemistry in diagnosis and prognosis / T.R. Helliwell-Electron microscopy : Use of lectin-peroxidase prior to embedding / A. Ellinger-Electron microscopy : Use of lectin-gold after embedding / R. Herken and B. Manshausen-Amplification of lectin-gold histochemistry / J.F. Madrld, F. Hernandez and J. Ballesta-Electron microscopic methods for the demonstration of lectin-binding sites in cancer cell lines / B.S. Mitchell and U. Schumacher-Application of sequential smith degradation to lectin blots / C.K. Ching-Blot analysis with lectins for the evaluation of glycoproteins in cultured cells and tissues / C. Zuber, W.P. Li and J. Roth-Characterization of hiv gp120 envelope glycoprotein by lectin analysis / G.J. Gram and J.E. Hansen-Use of lectins for characterization of o-linked glycans of herpes simplex virus glycoproteins / S. Olofsson and A. Bolmstedt-Use of lectin for detection of agalactosyl igg / N. Tsuchiya [and others]-Lectins for detection of altered glycosylation of circulating glycoproteins : Alpha-1-antitrypsin / Y. Aoyagi and H. Asakura-Detection of altered glycosylation of alpha-fetoprotein using lectin-affinity electrophoresis / K. Taketa, M. Liu and H. Taga-Use of lectin-affinity electrophoresis for quantification and characterization of glycoforms of alpha-1 acid glycoprotein / T.C. Bog-Hansen-Abo(h) blood group expression on circulating glycoproteins / T. Matsui and K. Titani-Lectin/antibody "sandwich" elisa for quantification of circulating mucin as a diagnostic test for pancreatic cancer / N. Parker-Quantification of intestinal mucins / J.D. Milton and J.M. Rhodes-Purification and characterization of human serum and secretory iga1 and iga2 using jacalin / M.A. Kerr [and others]-Use of lectins in affinity purification of hiv and siv envelope glycoproteins / G. Gilljam-T-cell receptor purification / K.P. Kearse-Use of monomeric, monovalent lectin derivatives for flow cytometric analysis of cell surface glycoconjugates / H. Kaku and N. Shibuya-Analysis of subcellular components by fluorescent-lectin binding and flow cytometry / R.M. Guasch and J.E. O'Connor-Lectins as tools for the purification of liver endothelial cells / D.E. Gomez and U.P. Thorgeirsson-The use of soybean agglutinin (sba) for bone marrow (bm) purging and hematopoietic progenitor cell enrichment in clinical bone marrow transplantation / A. Nagler, S. Morecki and S. Slavin-Combined lectin/monoclonal antibody purging of bone marrow for use in conjunction with autologous bone marrow transplantation in the treatment of multiple myeloma / E.G. Rhodes-Mechanisms and assessment of mitogenesis : An overview / D.C. Kilpatrick-Mitogenic effects of lectins on epithelial cells / L.G. Yu and J.M. Rhodes-Use of lectins as mitogens for lymphocytes / D.C. Kilpatrick-Effect of lectins on uptake of polyamines / S. Bardocz and A. White-Effects of lectins on cytoskeletal organization in mammalian cells / P. Carinci, E. Becchetti and M. Bodo-Effect of lectins on protein kinase activity / K. Sada and H. Yamamura-Lectin-induced calcium mobilization in human platelets : Use of fluorescent probes / G. Ramaschi and M. Torti-Lectin-triggered superoxide/h(2)o (2) and granule enzyme release from cells / A.V. Timoshenko, K. Kayser and H.J. Gabius-Cytotoxic effects of lectins / E. Gorelik-Application of the lectin-dependent cell-mediated cytotoxicity assay to bronchoalveolar lavage fluid and venous blood samples collected from canine lung allografts / A.G. Lee and H.A. Shennib-The effect of lectins on crypt cell proliferation in organ culture / S.D. Ryder-Effects of lectin ingestion on animal growth and internal organs / A. Pusztai-Lectin ingestion : Changes in mucin secretion and bacterial adhesion to intestinal tissue / H. Ceri, J.G. Banwell and R. Fang-Assessment of lectin inactivation by heat and digestion / A. Pusztai and G. Grant-Use of lectins as transganglionic neuronal tracers in the study of unmyelinated primary sensory neurons / M.B. Plenderleith and P.J. Snow-Lectin inhibition of bacterial adhesion to animal cells / M.W. Stinson and J.R. Wang-Inhibition of hiv infection by lectin binding to cd4 / J. Favero and V. Lafont-Inhibition of hiv infection by lectin binding to gp120 / A. Theresa and N. Mahmood-Absorption enhancement by lectin-mediated endo- and transcytosis / E. Haltner, G. Borchard and C.M. Lehr-The use of lectins for liposome targeting in drug delivery / M. Kaszuba and M.N. Jones |
| Summary |
Lectin Methods and Protocols brings together a comprehensive collection of optimized methods for using lectins in biomedical research. The techniques-fine-tuned by the researchers who developed them-apply lectins to the analysis of carbohydrates, to the isolation and quantification of glycoproteins, and to living cells. Also included are step-by-step methods for the separation of human bone marrow cells for clinical use, for the assessment of the biological effects of plant lectins, and for the use of light and electron microscopy in analyzing carbohydrate structures. Taken together, the methods detailed here offer extremely useful tools for examining structural changes in glycosylation and their functional consequences for human pathophysiology Lectin Methods and Protocols provides invaluable, easily reproducible techniques suitable for application to a wide range of important biomedical problems. The book will immediately become the standard practical resource and guide to lectin techniques for today's biomedical researchers |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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English |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| In |
Springer Protocols |
| Subject |
Lectins.
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Lectins -- Analysis
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Lectins -- analysis
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Lectins
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Lectins
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Lectins -- Analysis
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Biowissenschaften
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Analyse
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Aufsatzsammlung
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Lectine
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Molekularbiologie
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Lectinen.
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Wetenschappelijke technieken.
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Lectinas.
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Lectines.
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Lectines -- Analyse.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Rhodes, Jonathan M.
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Milton, Jeremy D.
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| ISBN |
9781592595938 |
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1592595936 |
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