| Description |
1 online resource |
| Contents |
Intro -- Preface -- Contents -- 1: The Physical Principles of Light Propagation and Light-Matter Interactions -- 1.1 Definitions of Light -- 1.2 Particle and Wave Properties of Light -- 1.3 Polarization -- 1.4 Refraction -- 1.5 Reflection -- 1.6 Light Scattering -- 1.7 Diffraction and Its Limits -- 1.8 Photon Absorption, Fluorescence, and Stimulated Emission -- 1.9 Filtering Light -- References -- Further Reading -- 2: Design, Alignment, and Usage of Infinity-Corrected Microscope -- 2.1 Development of Compound Microscope with Infinity Optics |
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2.2 Design Parameters: Resolution Limit, Numerical Aperture, Magnification, Depth of Field, and Field of View -- 2.3 Optical Aberrations and Their Corrections -- 2.4 Design Specifications of the Infinity-Corrected Microscope Objective -- 2.5 Critical and Köhler Illuminations -- 2.6 Components of Infinity-Corrected Microscope System -- 2.7 Alignment for Designing the Infinity-Corrected Bright Field Microscopy -- 2.8 Label-Free and Quantitative Phase Microscopy -- 2.8.1 Dark Field Microscopy -- 2.8.2 Zernike Phase Contrast Microscopy -- 2.8.3 Differential Interference Contrast (DIC) Microscope |
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2.8.4 Digital Holographic Microscopy for Quantitative Phase Measurement -- References -- 3: Epifluorescence Microscopy -- 3.1 Fluorescence -- 3.1.1 Jablonski Diagram -- 3.1.2 Fluorescent Markers -- 3.2 Fluorescence Microscope Setup -- 3.2.1 Excitation Module -- 3.2.2 Objective -- 3.2.3 Fluorescence Filters -- 3.2.4 Emission Module -- 3.3 Basics of Sample Preparation for Fluorescence Microscopy -- 3.4 Main Applications of Fluorescence Microscopy -- 3.4.1 Structural Imaging -- 3.4.2 Functional Imaging -- References -- Further Reading |
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4: Basic Digital Image Acquisition, Design, Processing, Analysis, Management, and Presentation -- 4.1 Image Acquisition and Analysis: Why they Are Needed -- 4.1.1 Image Relevance in Science from Biology to Astronomy -- 4.1.2 Need for Quantitative Methods Is Extended to Light Microscopy -- 4.2 Representation of Reality: Image Acquisition -- 4.2.1 What Is the Difference Between a Material Object and a Representation of It? -- 4.2.2 Light Microscopy Key Concepts -- 4.2.2.1 What Is Light Microscopy? -- 4.2.2.2 Optical Resolution -- 4.2.2.3 Microscope Elements -- 4.2.2.4 Fluorescence |
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4.2.2.5 Transmitted Light -- 4.2.2.6 Detectors: Cameras and PMT/Hybrid -- 4.2.2.7 Digitization -- 4.2.3 Examples of Microscope Systems: Simplified Light Path for Wide-Field and Confocal Laser Scanning Microscopy -- 4.2.4 Experiment Design -- 4.2.5 Questions and Answers -- 4.3 Image Visualization Methods -- 4.4 Image Analysis -- 4.4.1 Questions and Answers -- 4.4.2 Questions and Answers -- 4.5 Publication of Images and Data -- 4.5.1 Questions and Answers -- References -- Additional References -- 5: Confocal Microscopy -- 5.1 Introduction -- 5.2 Fluorescence Blurring |
| Summary |
This textbook is an excellent guide to microscopy for students and scientists, who use microscopy as one of their primary research and analysis tool in the laboratory. The book covers key microscopy principles and explains the various techniques such as epifluorescence microscopy, confocal/live cell imaging, SIM/light sheet microscopy, and many more. Easy-to-understand protocols provide helpful guidance for practical implementation in various commercially available imaging systems. The reader is introduced to histology and further be guided through advanced image acquisition, classification and analysis. The book is written by experienced imaging specialists from the UK, other EU countries, the US and Asia, and is based on advanced training courses for master students and PhD students. Readers are not expected to be familiar with imaging and microscopy technologies, but are introduced to the subject step by step. This textbook is indented for biomedical and medical students, as well as scientists and postdocs who want to acquire a thorough knowledge of microscopy, or gain a comprehensive overview of modern microscopy techniques used in various research laboratories and imaging facilities. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (Springerlink, viewed December 15, 2022) |
| Subject |
Microscopy.
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microscopy.
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Microscopy
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| Form |
Electronic book
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| Author |
Nechyporuk-Zloy, Volodymyr, editor
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| ISBN |
9783031044779 |
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3031044770 |
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