| Description |
1 online resource (xiv, 332 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 43 |
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Methods in molecular biology (Clifton, N.J.) ; v. 43
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| Contents |
Alternative method of assessing toxicity / Chris K. Atterwill -- LLC-RK1 cell screening test for nephrotoxicity / David J. White, Chris Seaman -- Preparation and use of cultured astrocytes for assay of gliotoxicity / Mark R. Cookson, R. McClean, Vietor W. Pentreatk -- Human thyroid culture / Carmel Mothersill -- Thyroid follicular cells in monolayer culture : in vitro models for thyroid toxicity testing / Stephen P. Bidey -- Dust toxicity in rat alveolar macrophage cultures / Yrjö Collan, Veli- Matti Kosma -- Hepatoma cell cultures as in vitro models for hepatotoxicity / Margherita Ferro -- Cytotoxicity, DNA fragmentation, and DNA repair synthesis in primary human hepatocytes / Giovanni Brambilla, Antonietta Martelli -- Model cavity method: invittox -- Human esophageal culture / Carmel Mothersill -- The application of in vitro models of anterior pituitary function in toxicity testing / Glenda E. Gillies, Julia C. Buckingham -- The application of in vitro models of hypothalamic function in toxicity testing / Glenda E. Gillies, Julia C. Buckingham -- The FRAME cytotoxicity test (kenacid blue) / Richard H. Clothier -- Allium test / Geirid Fiskesjö -- Measurement of cell membrane toxicity by means of 2-deoxy-D-glucose / Erik Walum, Anna Forsby -- MTT assays / Rosa Supino -- V79 cytotoxicity test for membrane damage / Vera Bianchi -- SIRC cytotoxicity test / Odile Blein-Sella, Monique Adolphe -- Rabbit articular chondrocyte functional toxicity test / Odile Blein-Sella, Monique Adolphe -- Balbh 3T3 cytotoxicity test / H.M. Liebsck, Horst Spielmann -- The pollen tube growth test / Udo Kristen, Rolf Kappler -- HET-CAM test / Horst Spielmann -- The use of the bovine isolated cornea as a possible in vitro test for ocular irritancy / Ann M. Northover -- Cytotoxicity in an anchorage-independent fibroblast cell line measured by a combination of fluorescent dyes / Richard B. Kemp -- Cell culture phototoxicity test / Paul A. Duffy -- The murine local lymph node assay / Jennifer Hilton, Ian Kimber -- Polymorphonuclear leukocyte locomotion / Matteo Valentino -- Immune function assays / Steve Nicklin -- The DNA alkaline unwinding genotoxicity test / Georg Bolcsfoldi -- Measurement of unscheduled DNA synthesis in vitro using primary rat hepatocyte cultures / Steve Dean -- Gene mutation assays in mammalian cells / Julie Clements -- Measurement of chromosome aberrations in vitro using human peripheral blood lymphocytes / Richard Marshall -- Mutation assays in bacteria / Christopher Clare -- Chick Embryotoxicity Screening Test (CHEST I and II) / Wendy J. Davies, Stuart J. Freeman -- Frog Embryo Teratogenesis Assay: Xenopus (FETAX) / Wendy J. Davies, Stuart J. Freeman -- The Drosophila melanogaster assay / Wendy J. Davies, Stuart J. Freeman -- The Hydra attentuata assay / Wendy J. Davies, Stuart J. Freeman |
| Summary |
In Vitro Toxicity Testing Protocols is a collection of proven, state-of-the-art methods that toxicologists can use in carrying out laboratory studies. The book's expert contributors offer a variety of efficient in vitro toxicology techniques that can be applied regularly in your lab, or can serve as the fundamental basis for the establishment of novel techniques in working laboratories. All the procedures are highly reliable. Also included are ex vivo techniques, whose development embraces the twin aims of reduction and refinement of the use of laboratory animals; the editors believe this is an important step toward the ethical treatment of laboratory animals. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, concise, easy-to-follow, step-by-step protocols are provided for each technique, together with extensive notes that cover troubleshooting and offer special tips to ensure the successful completion of your experiments |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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English |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
Toxicity testing -- In vitro -- Laboratory manuals
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Toxicity Tests -- methods
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Toxicity testing -- In vitro
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Toxicologie.
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Testen.
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Wetenschappelijke technieken.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
O'Hare, Sheila.
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Atterwill, C. K.
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| ISBN |
9781592595303 |
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1592595308 |
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159259526X |
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9781592595266 |
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