| Description |
1 online resource (xi, 352 pages) : illustrations |
| Series |
Methods in molecular biology ; 657 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 657
|
| Contents |
Protein antigen expression in escherichia coli for antibody production / D.M. Rancour, S.K. Backues and S.Y. Bednarek -- Expression of epitope-tagged proteins in plants / T. Furuichi -- Expression of epitope-tagged proteins in arabidopsis leaf mesophyll protoplasts / Y.H. Cho and S.D. Yoo -- Transient expression of epitope-tagged proteins in mammalian cells / M.L. Styers, J. Lowery and E. Sztul -- Production and purification of polyclonal antibodies / M. Nakazawa, M. Mukumoto and K. Miyatake -- Production and purification of monoclonal antibodies / M. Nakazawa, M. Mukumoto and K. Miyatake -- Production of antipeptide antibodies / B.S. Lee [and others] -- Preparation of colloidal gold particles and conjugation to protein A, IgG, F(ab')(2), and streptavidin / S. Yokota -- Immunoelectron microscopy of chemically fixed developing plant embryos / T. Osafune and S.D. Schwartzbach -- Pre-embedding immunogold localization of antigens in mammalian brain slices / T. Schikorski -- Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells / H. Hagiwara [and others] -- Immunoelectron microscopy of cryofixed and freeze-substituted plant tissues / M. Takeuchi, K. Takabe and Y. Mineyuki -- In vivo cryotechniques for preparation of animal tissues for immunoelectron microscopy / S. Ohno [and others] -- Immunoelectron microscopy of cryofixed freeze-substituted mammalian tissue culture cells / A. Sawaguchi -- Immunoelectron microscopy of cryofixed freeze-substituted saccharomyces cerevisiae / J. Fiserova and M.W. Goldberg -- High-resolution molecular localization by freeze-fracture replica labeling / A. Fujita and T. Fujimoto -- Pre-embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectin / Y. Akimoto and H. Kawakami -- Pre-embedding nanogold silver and gold intensification / A. Yamamoto and R. Masaki -- The post-embedding method for immunoelectron microscopy of mammalian tissues: A standardized procedure based on heat-induced antigen retrieval / S. Yamashita -- Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells / H. Hagiwara [and others] -- Serial section immunoelectron microscopy of algal cells / T. Osafune and S.D. Schwartzbach -- Freeze-etch electron tomography for the plasma membrane interface / N. Morone -- Localization of rDNA at nucleolar structural components by immunoelectron microscopy / S. Sato and Y. Sato -- Immunogold labelling for scanning electron microscopy / M.W. Goldberg and J. Fiserova -- Horseradish peroxidase as a reporter gene and as a cell-organelle-specific marker in correlative light-electron microscopy / T. Schikorski -- Monitoring rapid endocytosis in the electron microscope via photoconversion of vesicles fluorescently labeled with FM1-43 / T. Schikorski |
| Summary |
Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships |
| Analysis |
celbiologie |
|
cellular biology |
|
antilichamen |
|
antibodies |
|
levenswetenschappen |
|
life sciences |
|
Biology (General) |
|
Biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Electron microscopy.
|
|
Microscopy, Electron
|
|
Microscopy, Immunoelectron
|
|
electron microscopy.
|
|
Electron microscopy
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Schwartzbach, Steven D
|
|
Osafune, Tetsuaki
|
| ISBN |
9781607617839 |
|
1607617838 |
|
9781607617822 |
|
160761782X |
|
