Title Gene synthesis : methods and protocols / edited by Jean Peccoud

Published New York : Humana Press, ©2012

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Description 1 online resource (xi, 297 pages) Series Methods in molecular biology, 1064-3745 ; 852 Methods in molecular biology (Clifton, N.J.) ; v. 852. Contents PartI Assembly of oligonucleotides in synthons -- Building block synthesis using the polymerase chain assembly method / Julie A. Marchand and Jean Peccoud -- Oligonucleotide assembly in yeast to produce synthetic DNA fragments / Daniel G. Gibson -- Topdown real-time gene synthesis / Mo Chao Huang [and others] -- De novo DNA synthesis using single-molecule PCR / Tuval Ben Yehezekel, Gregory Linshiz, and Ehud Shapiro -- Part II Synthon assembly -- SLIC : a method for sequence- and ligation-independent cloning / Mamie Z. Li and Stephen J. Elledge -- Assembly of standardized DNA parts using bioBrick ends in E. coli / Olivia Ho-Shing [and others] -- Assembling DNA fragments by USER fusion / Narayana Annaluru [and others] -- Fusion PCR via novel overlap sequences / Kamonchai Cha-aim [and others] -- Using recombineering to generate point mutations : the oligonucleotide-based "Hit and Fix" method / Suhwan Chang, Stacey Stauffer, and Shyam K. Sharan -- Using recombineering to generate point mutations : galK-based positive-negative selection method / Kajal Biswas, Stacey Stauffer, and Shyam K. Sharan -- Assembling large DNA segments in yeast / Heloise Muller [and others] -- Recursive construction of perfect DNA molecules and libraries from imperfect oligonucleotides / Gregory Linshiz, Tuval Ben Yehezkel, and Ehud Shapiro -- Cloning whole bacterial genomes in yeast / Gwynedd A. Benders -- Production of infectious poliovirus from synthetic viral genomes / Jeronimo Cello and Steffen Muller -- Part III Software for gene synthesis -- In silico design of functional DNA constructs / Alan Villalobos, Mark Welch, and Jeremy Minshull -- Using DNAworks in designing oligonucleotides for PCR-based gene synthesis / David Hoover -- De novo gene synthesis design using Tmprime software / Mo-Huang Li [and others] -- Design-a-gene with GeneDesign / Sarah M. Richardson [and others] -- Part IV Education and security -- Leading a successful iGEM team / Wayne Materi -- Build-a-genome course / Eric M. Cooper [and others] -- DNA synthesis security / Ali Nouri and Christopher F. Chyba Summary The de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider Analysis Medicine Human genetics Genetic engineering Biomedicine biomedische wetenschappen humane genetica genetische modificatie Medicine (General) Geneeskunde (algemeen) Notes Includes index Bibliography Includes bibliographical references and index Notes English Subject Genetic engineering -- Laboratory manuals Bioinformatics. DNA replication. Genetics -- Technique. Genes, Synthetic DNA -- biosynthesis DNA Replication Genetic Techniques Synthetic Biology -- methods Computational Biology Genetics -- Technique DNA replication Bioinformatics Genetic engineering Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Peccoud, Jean LC no. 2012930137 ISBN 9781617795640 161779564X 1617795631 9781617795633

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