Title Microarrays. Volume 1, Synthesis methods / edited by Jang B. Rampal

Edition 2nd ed Published Totowa, N.J. : Humana, ©2007

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Description 1 online resource (xix, 448 pages) : illustrations Series Methods in molecular biology, 1064-3745 ; 381 Methods in molecular biology (Clifton, N.J.) ; v. 381 Contents Introduction: Array technology -- an overview / H. Seliger -- Current microarray surface chemistries / D.W. Grainger [and others] -- Nonfouling surfaces: A review of principles and applications for microarray capture assay designs / P. Gong and D.W. Grainger -- Optimization of oligonucleotide DNA microarrays / M. Dufva and C.B. Christensen -- Detection of DNA copy-number alterations in complex genomes using array comparative genomic hybridization / W.W. Cai -- Evaluating the quality of data from DNA microarray measurements / L. Wang [and others] -- Construction of oligonucleotide microarrays (biochip) using heterobifunctional reagents / J. Choithani [and others] -- Choice of polymer matrix, its functionalization and estimation of functional group density for preparation of biochips / S. Mahajan [and others] -- Methods in high-resolution, array-based comparative genomic hybridization / M.R. McCormick, R.R. Selzer and T.A. Richmond -- Design and fabrication of spotted long oligonucleotide microarrays for gene expression analysis / C.C. Chou and K. Peck -- Construction of in situ oligonucleotide arrays on plastic / J.B. Rampal, P.J. Coassin and R.S. Matson -- Detecting ligated fragments on oligonucleotide microarrays: Optimizing chip design, array multiplex ligation-dependent probe amplification modification, and hybridization parameters / I.R. Berry, C.A. Delaney and G.R. Taylor -- Detection of single-nucleotide polymorphisms in cancer-related genes by minisequencing on a microelectronic DNA chip / A. Ho-Pun-Cheung [and others] -- Hybridization analysis using oligonucleotide probe arrays / R.S. Matson and J.B. Rampal -- In situ synthesis of peptide microarrays using ink-jet microdispensing / B.V. Antohe and P.W. Cooley -- Intein-mediated peptide arrays for epitope mapping and kinase/phosphatase assays / M.Q. Xu [and others] -- Printing low density protein arrays in microplates / R.S. Matson [and others] -- Forward-phase and reverse-phase protein microarray / Y. Zong [and others] -- Cell microarray for functional exploration of genomes / D. Castel [and others] -- Quantification of mixed-phase hybridization on polymer microparticles by europium(III) ion fluorescence / K. Ketomaki and H. Lonnberg -- Measurement of the sugar-binding specificity of lectins using multiplexed bead-based suspension arrays / K. Yamamoto, F. Yasukawa and S. Ito -- Nanotechnology: Moving from microarrays toward nanoarrays / H. Chen and J. Li Summary Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology Analysis analytische methoden analytical methods bindende eiwitten binding proteins bindingsplaatsen binding sites biologische technieken biological techniques microarrays dna microarrays protocollen protocols Molecular Biology (General) Moleculaire biologie (algemeen) Notes Earlier edition published as DNA arrays. Totowa, N.J. : Humana, 2001 Bibliography Includes bibliographical references and indexes Notes Print version record Subject DNA microarrays. Protein microarrays. Oligonucleotide Array Sequence Analysis Protein Array Analysis SCIENCE -- Life Sciences -- Genetics & Genomics. DNA microarrays Protein microarrays Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Rampal, Jang B. Rampal, Jang B. DNA arrays ISBN 159745303X 9781597453035 1588295893 9781588295897

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