| Description |
1 online resource (xi, 235 pages) : illustrations (some color) |
| Series |
Methods in molecular medicine ; 1326 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1326. 1064-3745
|
| Contents |
Celiac disease : background and historical context / Graham D. Turner, Margaret R. Dunne, and Anthony W. Ryan -- Celiac disease : diagnosis / Greg Byrne and Conleth F. Feighery -- Generating transgenic mouse models for studying celiac disease / Josephine M. Ju, Eric V. Marietta, and Joseph A. Murray -- Study designs for exploring the non-HLA genetics in celiac disease / Åsa Torinsson Naluai -- Twenty-four hour ex vivo culture of celiac duodenal biopsies / Sarah E.J. Cooper, Sharon Wilson, and Conleth F. Feighery -- Isolation and cloning of gluten-specific T cells in celiac disease / Yvonne Kooy-Winkelaar and Frits Koning -- Flow cytometric analysis of human small intestinal lymphoid cells / Margaret R. Dunne -- Adaptation of a cell-based high content screening system for the in-depth analysis of celiac biopsy tissue / Sarah E.J. Cooper [and others] -- HLA genotyping : methods for the identification of the HLA-DQ2,-DQ8 heterodimers implicated in celiac disease (CD) susceptibility / Maria Edvige Fasano, Ennia Dametto, and Sandra D'Alfonso -- Detecting allelic expression imbalance at candidate genes using 5' exonuclease genotyping technology / Jillian M. Gahan [and others] -- Gene expression profiling of celiac biopsies and peripheral blood monocytes using taqman assays / Martina Galatola, Renata Auricchio, and Luigi Greco -- Cloning gene variants and reporter assays / Ben Molloy and Ross McManus -- Epigenetic methodologies for the study of celiac disease / Antoinette S. Perry, Anne-Marie Baird, and Steven G. Gray -- Candidate gene knockdown in celiac disease / Ben Molloy [and others] -- Perl one-liners : bridging the gap between large data sets and analysis tools / Karsten Hokamp -- Bioinformatic analysis of antigenic proteins in celiac disease / Cathal P. O'Brien -- Quality control procedures for high-throughput genetic association studies / Ciara Coleman, Emma M. Quinn, and Ross McManus -- Quality control and analysis of NGS RNA sequencing data / Emma M. Quinn and Ross McManus |
| Summary |
This volume provides detailed molecular analysis of the associated pathways and cell types involved in celiac disease, and embraces new technologies such as next generation sequencing. Authoritative and thorough, the book brings together novel and more traditional methods in molecular biology and immunology in order to provide a tool-kit for all stages for celiac disease research - from the practicalities of identifying, obtaining, stabilising and maintaining high quality samples, to molecular analysis and bioinformatics |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Celiac disease -- Molecular aspects -- Laboratory manuals
|
|
Genetics -- Technique.
|
|
Celiac Disease -- immunology
|
|
Celiac Disease -- genetics
|
|
Genetic Techniques
|
|
Immunologic Techniques
|
|
Immunology.
|
|
Medical -- Immunology.
|
|
Genetics -- Technique
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Ryan, Anthony W., editor
|
| LC no. |
2015944312 |
| ISBN |
9781493928392 |
|
1493928392 |
|
1493928384 |
|
9781493928385 |
|
