| Description |
1 online resource (x, 191 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 1392 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1392
|
| Contents |
Targeted Q-PCR-based method for point mutation testing by analyzing circulating DNA for cancer management care / Alain R. Thierry -- COLD-PCR : applications and advantages / Zhuang Zuo and Kausar J. Jabbar -- PCR-based detection of DNA copy number variation / Meenakshi Mehrotra -- Emulsion PCR : techniques and applications / Rashmi Kanagal-Shamanna -- Digital PCR : principles and applications / Rashmi Kanagal-Shamanna -- Quantitative PCR for plasma epstein-barr virus loads in cancer diagnostics / Sanam Loghavi -- High-resolution melt curve analysis in cancer mutation screen / Meenakshi Mehrotra and Keyur P. Patel -- Locked nucleic acid probes (LNA) for enhanced detection of low-level, clinically significant mutations / Khedoudja Nafa, Meera Hameed, and Marie E. Arcila -- Genotyping of frequent mutations in solid tumors by PCR- based single-base extension and MassARRAY analysis / Alberto Paniz Mondolfi and Rajesh R. Singh -- Microfluidics-based PCR for fusion transcript detection / Hui Chen -- Polymerase chain reaction diagnosis of Leishmaniasis : a species-specific approach / Eglys González-Marcano [and others] -- Detection of Trypanosoma cruzi by Polymerase Chain Reaction / María Elizabeth Márquez [and others] -- PCR techniques in next-generation sequencing / Rashmi S. Goswami -- Single-cell quantitative PCR : advances and potential in cancer diagnostics / Chi Young Ok, Rajesh R. Singh, and Alaa A. Salim -- Quantitative real-time PCR : recent advances / Charanjeet Singh and Sinchita Roy-Chowdhuri -- PCR techniques in characterizing DNA methylation / Khalida Wani and Kenneth D. Aldape |
| Summary |
This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routineclinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successfulMethods in Molecular Biologyseries, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Clinical Applications of PCR, Third Editionserves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Polymerase chain reaction -- Diagnostic use.
|
|
Polymerase Chain Reaction -- methods
|
|
Cellular biology (cytology)
|
|
Science -- Life Sciences -- Biology -- Molecular Biology.
|
|
Polymerase chain reaction -- Diagnostic use
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Luthra, Rajyalakshmi, editor.
|
|
Singh, Rajesh R., editor.
|
|
Patel, Keyur P., editor.
|
| ISBN |
9781493933600 |
|
1493933604 |
|
1493933582 |
|
9781493933587 |
|
