Description |
1 online resource (streaming video file) (691 secs.) |
Series |
Immunology and Infection |
Summary |
Here, we present a protocol to isolate and cultivate murine peritoneal mast cells. We also describe two protocols for their functional characterization: a fluorescent imaging of intracellular free Ca2+ concentration and a degranulation assay based on colorimetric quantification of the released β-hexosaminidase |
Audience |
For undergraduate, graduate, and professional students |
Notes |
English |
|
Description based on online resource; title from resource description page |
Subject |
Immunology and Infection
|
Form |
Streaming audio
|
|