Limit search to available items
Book Cover
E-book

Title MicroRNA from bench to bedside / eidted by Junjie Xiao
Published London : Academic Press, 2022

Copies

Description 1 online resource
Contents Front Cover -- MicroRNA -- Copyright Page -- Contents -- List of contributors -- Preface -- I. Overview -- 1 Overview of micro-RNA -- 1.1 Introduction -- 1.2 The biogenesis of micro-RNA -- 1.3 Roles of micro-RNA in cancer -- 1.3.1 micro-RNA in bladder cancer -- 1.3.2 micro-RNA in breast cancer -- 1.3.3 micro-RNA in liver cancer -- 1.3.4 micro-RNA in melanoma -- 1.3.5 micro-RNA in pancreatic cancer -- 1.3.6 micro-RNA in colorectal cancer -- 1.3.7 micro-RNAs and endometrial cancer -- 1.3.8 micro-RNAs and renal cancer -- 1.4 micro-RNAs and chemoresistance/chemosensitivity -- 1.5 micro-RNA and SARS-CoV-2 -- 1.6 micro-RNA in immune system -- 1.7 micro-RNA and autoimmune -- 1.8 micro-RNA and aging -- 1.9 micro-RNAs and other diseases -- 1.10 micro-RNA biogenesis of plant -- 1.11 Cross talking of micro-RNA -- 1.12 Conclusion -- References -- II. Biogenesis and maturation -- 2 Maturation of microRNAs -- 2.1 Introduction -- 2.2 The classic canonical miRNA maturation pathway -- 2.2.1 Transcription of miRNAs -- 2.2.2 Biogenesis and maturation of miRNA -- 2.2.2.1 Canonical pathway -- 2.2.2.1.1 miRNA transcription in the nucleus -- 2.2.2.1.2 Export to the cytoplasm -- 2.2.2.1.3 Maturation of miRNA in the cytoplasm -- 2.2.2.1.4 RNA-induced silencing complex-loading machine -- 2.3 The alternative noncanoniocal miRNA maturation pathway -- 2.3.1 Mirtrons -- 2.3.2 Dicer-independent miRNAs -- 2.3.3 Small nucleolar RNA-derived miRNAs -- 2.4 The commonly used approaches to study miRNA maturation -- 2.4.1 Sample types and miRNA extraction -- 2.4.2 Quality and quantity assessment -- 2.4.3 miRNA profiling methods -- 2.4.3.1 Quantitative reverse transcription-PCR-based methods -- 2.4.3.2 Hybridization-based methods -- 2.4.3.3 RNA-Seq -- References -- III. Methodology and bioinformatics -- 3 MicroRNA interference -- 3.1 Background: definition and applications
3.2 Methods -- 3.2.1 miRNA inhibition -- 3.2.1.1 miRNA inhibition by oligonucleotides -- 3.2.1.2 miRNA inhibition by target masking -- 3.2.1.3 miRNA inhibition by miRNA sponges -- 3.2.1.4 miRNA inhibition by drug-like small molecules -- 3.2.1.5 miRNA inhibition by genome editing -- 3.2.2 miRNA upregulation -- 3.2.3 Delivery methods -- 3.2.3.1 Nonviral-based delivery -- 3.2.3.2 Virus-based delivery -- 3.2.4 miRNA interference-based therapeutics -- 3.3 Conclusion -- References -- 4 MicroRNA target prediction and validation -- 4.1 Introduction -- 4.2 Biological concepts used in miRNA target predictions -- 4.2.1 miRNA seed region binding -- 4.2.2 Free energy -- 4.2.3 Evolutionary conservation -- 4.2.4 Target site accessibility -- 4.2.5 Target site abundance -- 4.3 Available miRNA target prediction algorithms -- 4.4 Common experimental validation methods of miRNA targets -- 4.5 Machine learning algorithms to learn complex patterns in miRNA-target interactions -- 4.6 Other useful resources and databases for miRNA studies -- 4.7 Challenges and limitations in computational identification of miRNA targets -- 4.8 Concluding remarks and perspectives -- References -- 5 Turning data to knowledge: online tools, databases, and resources in microRNA research -- 5.1 Human miRNA regulation -- 5.2 The scope and organization of the chapter -- 5.3 Repositories for miRNA: catalogs and genome browsers -- 5.3.1 miRNA gene catalogs -- 5.3.2 miRNAs in genomic browsers -- 5.4 Gateway for miRNAs: integrative platforms -- 5.5 miRNA gene regulation: transcription factors and cellular context -- 5.6 miRNA-targets prediction: experiments and validations -- 5.6.1 miRNA-target prediction tools and resources -- 5.6.2 miRNA-target prediction validation databases -- 5.7 miRNA-target databases: network and pathways -- 5.8 miRNA sponge: ceRNA and lncRNA interaction
5.9 Genomic miRNA database: variations and isomiRs -- 5.10 miRNA dysregulation: diseases, cancer, and signaling -- 5.10.1 Disease-related miRNA databases -- 5.10.2 Cancer-related miRNA databases -- 5.11 Summary and future perspectives -- References -- IV. Molecular mechanisms and gene regulation -- 6 Function of microRNAs in the cytoplasm -- 6.1 Introduction -- 6.2 Subcellular localization of miRNAs -- 6.2.1 Processing bodies -- 6.2.2 Endoplasmic reticulum -- 6.2.3 Mitochondria -- 6.2.4 The nucleus -- 6.2.5 Other subcellular sites within the cytoplasm -- 6.3 Repression of gene expression via mRNA degradation -- 6.3.1 miRNA-mRNA binding -- 6.3.2 miRNA target cleavage -- 6.3.3 Target RNA destabilization through deadenylation and decapping -- 6.3.4 miRNA stability -- 6.4 Repression of gene expression via translational repression -- 6.4.1 Reduced translation initiation -- 6.4.2 Ribosome dissociation and reduced ribosome occupancy -- 6.5 Enhancement of gene expression -- 6.6 miRNA regulation of gene expression is cell context-specific -- 6.7 Conclusions -- References -- 7 MicroRNA turnover and nuclear function -- 7.1 Introduction -- 7.2 miRNA biogenesis -- 7.2.1 Canonical miRNA biogenesis: nuclear components -- 7.2.2 Noncanonical miRNA biogenesis -- 7.3 Nucleocytoplasmic shuttling of pre-miRNA -- 7.3.1 Exportin-5 (XPO5)-RanGTP carrier-mediated transport of canonical miRNAs -- 7.3.2 Exportin-1 (CRM1 -- XPO1) mediated transport -- 7.4 Nuclear localization of processing proteins and miRNAs -- 7.4.1 miRNA-induced silencing complex in the nucleus -- 7.4.2 miRNA localization in the nucleus -- 7.4.3 miRNA biogenesis in the nucleus -- 7.5 miRNA functions in the nucleus -- 7.5.1 Transcriptional regulation -- 7.5.2 Long noncoding RNAs regulation -- 7.5.3 Putative emerging roles of nuclear miRNAs -- 7.6 miRNA turnover -- 7.6.1 Variation in the stability of miRNAs
7.6.2 Mechanisms that control miRNA stability and degradation -- 7.6.2.1 3′ tails and 5′ capping of pre and mature miRNAs -- 7.6.2.2 3′ methylation of mature miRNAs -- 7.6.2.3 Intrinsic miRNA sequences and editing -- 7.6.2.4 AGO and GW182 in mature miRNA stability -- 7.6.2.5 Target-mediated miRNA protection -- 7.6.2.6 Target-directed miRNA degradation -- 7.6.2.7 Exoribonucleases involved in miRNA degradation -- 7.7 Conclusions -- References -- 8 MicroRNA-mediated transcriptional and posttranscriptional regulation -- 8.1 Introduction -- 8.2 Biogenesis of microRNAs -- 8.3 Regulatory function of nuclear microRNA -- 8.4 Regulatory function of mitochondrial microRNA -- 8.5 Transcriptional gene regulation mediated by nuclear microRNAs -- 8.5.1 microRNA transcriptional gene activation -- 8.5.2 microRNA transcriptional gene silencing -- 8.6 microRNAs regulate transcription by interaction with gene promoters -- 8.7 Conclusion -- References -- 9 Epigenetic regulation and microRNA expression -- 9.1 Introduction -- 9.2 microRNAs -- 9.3 Epigenetic regulation of microRNAs -- 9.3.1 DNA-methylation-mediated regulation of microRNAs -- 9.3.2 Histone modification-mediated regulation of microRNAs -- 9.3.3 RNA modification-mediated regulation of microRNAs -- 9.4 microRNAs affect epigenetic expression -- 9.4.1 DNA methylation and RNA modification regulated by microRNAs -- 9.4.2 Histone modification regulated by microRNAs -- 9.5 microRNAs-epigenetic regulator drugs -- 9.6 Conclusion and future prospective -- References -- 10 RNA m6A modification and microRNAs -- 10.1 Overview of epitranscriptome -- 10.1.1 Pseudouridine -- 10.1.2 N1-methyladenosine -- 10.1.3 ribose methylations -- 10.1.4 5-methylcytidine -- 10.1.5 N7-methylguanosine -- 10.1.6 N4-acetylcytidine -- 10.1.7 m6A -- 10.2 m6A and miRNA -- 10.2.1 m6A affects biogenesis of miRNA
10.2.2 m6A affects miRNA efficiency toward target mRNAs -- 10.2.3 miRNA can affect m6A -- 10.3 Conclusions -- References -- 11 Unconventional functions of miRNAs -- 11.1 Unconventional subcellular localizations of miRNAs -- 11.1.1 Nucleus -- 11.1.2 Nucleolus -- 11.1.3 Mitochondria -- 11.2 Unconventional miRNA functions: activation of innate immune sensors -- 11.2.1 Innate immune RNA sensors -- 11.2.1.1 Retinoic acid-inducible gene-like receptors -- 11.2.1.2 Toll-like receptors -- 11.2.2 Endogenous RNA sensing -- 11.2.2.1 Activation of innate immune receptors by host RNAs in virus-infected cells -- 11.2.2.2 Activation of RNA receptors in sterile conditions -- 11.2.3 miRNAs as ligands of innate immune sensors -- 11.2.3.1 Biogenesis of extracellular miRNAs -- 11.2.3.2 miRNA delivery to innate immune receptors -- 11.2.3.3 Features of innate immune receptor activation by miRNAs -- 11.2.3.4 The "cell-to-cell communication" theory: myth or reality? -- 11.2.4 miRNAs as Toll-like receptor-activators in pathology -- 11.2.4.1 Cancer -- 11.2.4.2 Neuron activation and death -- 11.2.4.3 Autoimmune diseases -- 11.2.4.4 Infectious diseases -- 11.2.4.5 Other diseases -- 11.3 Other unconventional miRNA functions -- 11.3.1 Activation of transcription -- 11.3.2 Activation of mRNA translation -- 11.3.3 miRNAs encoding for peptides -- 11.3.4 Interaction with non-AGO proteins -- Conflict of interest statement -- References -- V. MicroRNA as biomarkers -- 12 Detection methodologies for microRNA biomarker profiling -- 12.1 Introduction -- 12.2 miRNA extraction methods -- 12.3 miRNA profiling methods -- 12.3.1 Probe-hybridization unamplified techniques -- 12.3.1.1 Northern blot -- 12.3.1.2 In situ hybridization -- 12.3.1.3 RNA microarray -- 12.3.2 Amplification-based methods -- 12.3.2.1 Real-time quantitative PCR -- 12.3.2.2 Next-generation sequencing
Notes Includes index
Print version record
Subject MicroRNA.
MicroRNA -- Health aspects
MicroRNA.
Form Electronic book
Author Xiao, Junjie
ISBN 9780323885584
0323885586