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Author Zhuang, Bin, author

Title Development of a fully integrated sample-in-answer-out system for automatic genetic analysis / Bin Zhuang
Published Singapore : Springer, 2018


Description 1 online resource
Series Springer theses
Springer theses.
Contents Chapter 1: Introduction; 1.1 Genetic Analysis; 1.1.1 DNA Extraction; 1.1.2 DNA Amplification; 1.1.3 DNA Detection; Nucleic Acid Electrophoresis; DNA Microarray; Quantitative PCR; Gene Sequencing; 1.2 Microfluidic; 1.2.1 Silicon-Based Materials; 1.2.2 Paper-Based Materials; 1.2.3 Polymers-Based Material; 1.3 Microfluidic for Integrated Gene Detection; 1.3.1 Micrfluidic for DNA Extraction; 1.3.2 On-Chip Amplification; 1.3.3 Capillary Electrophoresis Chip; 1.3.4 Integrated Genetic Analysis System1.4 The Purpose and Significance of This Study; 1.5 Main Lines of Each Chapter; References; Chapter 2: Construction of a General Platform for Capillary Electrophoresis; 2.1 Chapter Introduction; 2.2 Construction of Capillary Electrophoresis Instrument; 2.2.1 Optical System; DNA Fluorescence Detection; Optical Design; Mechanical Design and Adjustment; 2.2.2 Circuit Board Sets; Scanning Stage Control; High-Voltage Module; Signal Processing Module; 2.2.3 Chip Loading Stage; 2.2.4 Industrial Design; 2.2.5 Software and Operation Process2.2.5.1 Start; Scan Chip; Find Channels; Operation; Results; Exit; 2.3 Capillary Electrophoresis Chip; 2.3.1 Design of Glass Capillary Electrophoresis Chip; 2.3.2 Manufacture of Capillary Electrophoresis Chip; 2.4 Instrument Verification; 2.4.1 PCR Amplification; 2.4.2 Pre-treatment of Glass Electrophoresis Chip; 2.4.3 Test Results; 2.5 Chapter Summary; References; Chapter 3: Integrated Module for Automated DNA Extraction and Amplification; 3.1 Chapter Introduction; 3.2 Integrated Chip for DNA Extraction and Amplification3.2.1 On-Chip Valve Structure; 3.2.2 Manufacture of DNA Extraction and Amplification Chip; 3.2.3 Verification on the On-Chip Valve Structure; 3.3 DNA Extraction and Amplification Equipment; 3.4 Calibration of the Integrated Sample Preparation System; 3.4.1 Calibration of PCR Temperature Curve; 3.4.2 The Automated Extraction and Amplification Process; 3.5 Verification of Automated DNA Extraction and Amplification System; 3.6 Screening of Congenital Hearing Loss Mutations Using Automated Sample Preparation System; 3.6.1 Experimental Setup; 3.6.2 Experimental Results3.7 Chapter Summary; References; Chapter 4: Fully Integrated Genetic Analysis System; 4.1 Chapter Introduction; 4.2 Design and Construction of the Fully Integrated Interface; 4.2.1 Deign of the Interface; 4.2.2 Design of Sample Electrode; 4.2.3 Verification of the S Electrode Design; 4.3 Upgrade of the Fully Integrated Chip; 4.4 Upgrade of the Integrated Instrument; 4.4.1 Upgrade of Instrument Design and Structure; 4.4.2 Upgrade of the Automated Operations; 4.5 Verification of the Fully Integrated System; 4.5.1 Verification of the Integrated Interface
Summary This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an "in-situ" PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system?s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid "sample-in-answer-out" testing in routine clinical practice
Bibliography Includes bibliographical references at the end of each chapters
Subject DNA -- Analysis
DNA -- analysis
SCIENCE -- Life Sciences -- Biochemistry.
DNA -- Analysis.
Form Electronic book
ISBN 9789811047534