| Description |
xi, 344 pages : illustrations ; 24 cm |
| Series |
Advances in molecular biology |
|
Advances in molecular biology.
|
| Contents |
Machine derived contents note: 1. The two-hybrid system: A personal view, S. Fields and P.L. Bartel -- I. The Two-Hybrid System -- 2. Searching for interacting proteins with the two-hybrid system I, C. Bai and S.J. Elledge -- 3. Searching for interacting proteins with the two-hybrid system II, A.B. Vojtek, J.A. Cooper, and S.M. Hollenberg -- 4. Searching for interacting proteins with the two-hybrid system III, E.A. Golemis and R. Brent -- 5. Constructing an activation domain fusion library, L. Zhu, D. Gunn, and S. Kuchibhatla -- II. Analyzing Interactions with the Two-Hybrid System -- 6. Obtaining structural information about protein complexes with the two-hybrid system, D.C. Amberg and D. Botstein -- 7. The reverse two-hybrid system, M. Vidal -- 8. Dissecting the biological relevance of multiple interactions, M.A. White -- 9. Investigation of ligand/receptor interactions and the formation of ternary complexes, B.A. Ozenberger and K.H. Young -- 10. Two-hybrid analysis of protein-protein interactions in the yeast pheromone response pathway, G.F. Sprague, Jr. and J.A. Printen -- 11. Two-hybrid screening and the cell cycle, G.J. Hannon -- 12. Two-hybrid analysis of genetic regulatory networks, R.L. Finley, Jr. and R. Brent -- III. Variations on the Two-Hybrid Theme -- 13. Mammalian cells as hosts for two-hybrid studies of protein-protein interaction, J.T. Tsan, Z. Wang, J. Jin, L.-Y. Hwang, R.O. Bash, and R. Baer -- 14. Detection of protein-protein interactions dependent on post-translational modifications, M.A. Osborne, M. Lubinus, and J.P. Kochan -- 15. Protein interactions mediated by small molecule ligands, V. Berlin -- 16. Use of a combinatorial peptide library in the two-hybrid assay, M. Yang -- IV. Related Methodologies -- 17. Isolation of DNA-binding proteins using a one-hybrid genetic screen, J.A. Chong and G. Mandel -- 18. A three-hybrid system to detect and analyze RNA-protein interactions in vivo, B. Zhang, B. Kraemer, D. SenGupta, S. Fields, and M. Wickens -- 19. Split ubiquitin: a sensor of protein interactions in vivo, N. Johnsson and A. Varshavsky |
| Analysis |
protein binding protein interactions |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Plasmids.
|
|
Protein binding -- Research -- Methodology.
|
|
Yeast fungi.
|
| Author |
Bartel, Paul L.
|
|
Fields, Stanley.
|
| LC no. |
96039040 |
| ISBN |
0195109384 |
|
