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Book Cover
Book
Author Rao, Beedu Sashidhar.

Title Experimental biochemistry : a student companion / Beedu Sashidhar Rao, Vijay Deshpande
Published Tunbridge Wells, Kent : Anshan, 2006

Copies

Location Call no. Vol. Availability
 W'PONDS  612.015 Rao/Eba  AVAILABLE
Description xiv, 302 pages : illustrations (some color) ; 24 cm
Contents Machine derived contents note: 1. Laboratory Safety and Hygiene I -- Chemical hazards 2 -- Biological hazards 3 -- Physical hazards 3 -- Electrical & Mechanical hazards 4 -- Standard operating procedures (SOP) for laboratory safety 5 -- Hazard management in the laboratory 6 -- Hazard symbols 10 -- Laboratory waste disposal 11 -- 2. Units and Measurements 13 -- Mass 13 -- Volume 13 -- Concentration of analyte expressed per unit volume 14 -- Concentration of analyte or solution expressed as percent 16 -- Density & specific gravity 17 -- Ionic strength 17 -- 3. Basic Statistical Concepts for Biochemical Analysis 19 -- Precision 19 -- Accuracy 19 -- Compntation of mean, standard deviation, -- coefficient of variation and standard error 21 -- 4. Basic Concepts and Use of Instruments in -- Biochemical Analysis 25 -- Photometry and spectrophotometry 25 -- Laboratory centrifuge 32 -- Electrophoresis 35 -- 5. pH and Buffers 39 -- pH. scale 40 -- pli meter 42 -- Buffers 43 -- 6. Qualitative Analysis 49 -- Amino acids and proteins 49 -- Carbohydrates 62 -- Lipids & steroids 72 -- 7. Quantitative Analysis 81 -- Titrimetric experiments 82 -- Spectrophotometric and photometric experiments 99 -- 8. Biochemical Separation Techniques 129 -- 9. Biochemical Preparations 153 -- 10. Enzyme Assays 173 -- Enzyme classification 173 -- In its of enzyme activity 174 -- Measurement of enzyme activity 175 -- 11. Clinical Biochemistry 191 -- Blood analysis 19! -- C iiical enozymoiogy 2 15 -- Gastric secretion 225 -- r iine aal isis 227 -- 12. Immunochemical Methods 245 -- 13. Food Analysis 265 -- Qualitative analysis of some common food adulterants 266 -- Quantitative analysis: Proximate analysis 269 -- Anal sis of anti-nutritional factors 276 -- Appendix 283 -- A. Physical constants 283 -- B. Visible light absorption, ilters and complementary hues 283 -- C. Boiling point & density of some organic solvents -- routinely used in the laboratory 284 -- D Mo ar absorption coefficients of selected biochemicals 285 -- E. Molanty, normality. % concentration and specific -- gravity of some acids and alkalies 28 -- ' Preparation of acid and alkali (1 N) solutions 286 -- C pH indicators for volumetric analysis 287 -- HI. Standard buffers for pHI meter calibration 87 -- 1. p w values (approximate value) of frequently used -- 0.1 M solutions 288 -- J. Amino acids: Abbreviations, molecular weight, -- isoelectric point & solubility 289 -- K. Molecular weight of certain proteins 290 -- L. Isoelectric point of few proteins 290 -- M. Determination of protein concentration by -- UV- absorption method 291 -- N. Sapni i cation, iodine & acid values of some -- common oils & fats 292 -- O. Determination of nucleic acid concentration by -- UV- absorption method 292 -- P Some nor ma human clinical chemistry values of -- blood, serum & urine 293 -- Q, Some useful web sites 296
Bibliography Includes bibliographical references and index
Subject Biochemistry -- Laboratory manuals.
Genre/Form Laboratory manuals.
Author Deshpande, Vijay.
LC no. 2006462911
ISBN 1904798519 (paperback)
9781904798514 (paperback)