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Book Cover
Book
Author Old, R. W.

Title Principles of gene manipulation : an introduction to genetic engineering / R.W. Old, S.B. Primrose
Edition Fifth edition
Published Oxford ; Boston : Blackwell Scientific, 1994

Copies

Location Call no. Vol. Availability
 W'PONDS  660.65 Old/Pog 1994  AVAILABLE
Description vi, 474 pages : illustrations ; 25 cm
Series Studies in microbiology
Studies in microbiology (Unnumbered)
Contents 1. Gene manipulation: an all-embracing technique -- 2. Basic techniques -- 3. Cutting and joining DNA molecules -- 4. Plasmids as cloning vehicles for use in E. coli -- 5. Bacteriophage and cosmid vectors for E. coli -- 6. Cloning strategies, gene libraries and cDNA cloning -- 7. Recombinant selection and screening -- 8. Expression in E. coli of cloned DNA molecules -- 9. Analysing DNA sequences -- 10. The polymerase chain reaction -- 11. Changing genes: site-directed mutagenesis -- 12. Cloning in bacteria other than E. coli -- 13. Cloning in Saccharomyces cerevisiae and other microbial eukaryotes -- 14. Gene transfer to plants -- 15. Introducing genes into animal cells -- 16. Transferring genes into animal oocytes, eggs, embryos and specific animal tissues -- 17. The impact of recombinant DNA technology: the generation of novelty -- Appendix 1: Restriction endonuclease target sites -- Appendix 2: The genetic code and single-letter amino acid designations
Summary As in previous editions, the basic philosophy of this extremely popular text remains to present the principles and techniques of gene manipulation in sufficient detail for the non-specialist reader to understand them. However, in the five years since the last edition, molecular biologists have seen a dramatic increase in available techniques and applications. Every chapter in this fifth edition has been extensively revised or rewritten to take these developments into account - including a greatly expanded section on the polymerase chain reaction and its manifold applications; a new chapter examining the impact of recombinant DNA techniques on our understanding of human genetics; and extended coverage of gene transfer techniques. With entirely new illustrations in two colours throughout, this will remain the textbook of choice for undergraduates and postgraduates alike
Notes Includes index
Bibliography Includes bibliographical references (pages 412-464) and index
Subject Recombinant DNA.
Genetic engineering.
DNA, Recombinant.
Genetic Engineering.
Author Primrose, S. B.
LC no. 94000932
ISBN 0632037121