Description |
vi, 474 pages : illustrations ; 25 cm |
Series |
Studies in microbiology |
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Studies in microbiology (Unnumbered)
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Contents |
1. Gene manipulation: an all-embracing technique -- 2. Basic techniques -- 3. Cutting and joining DNA molecules -- 4. Plasmids as cloning vehicles for use in E. coli -- 5. Bacteriophage and cosmid vectors for E. coli -- 6. Cloning strategies, gene libraries and cDNA cloning -- 7. Recombinant selection and screening -- 8. Expression in E. coli of cloned DNA molecules -- 9. Analysing DNA sequences -- 10. The polymerase chain reaction -- 11. Changing genes: site-directed mutagenesis -- 12. Cloning in bacteria other than E. coli -- 13. Cloning in Saccharomyces cerevisiae and other microbial eukaryotes -- 14. Gene transfer to plants -- 15. Introducing genes into animal cells -- 16. Transferring genes into animal oocytes, eggs, embryos and specific animal tissues -- 17. The impact of recombinant DNA technology: the generation of novelty -- Appendix 1: Restriction endonuclease target sites -- Appendix 2: The genetic code and single-letter amino acid designations |
Summary |
As in previous editions, the basic philosophy of this extremely popular text remains to present the principles and techniques of gene manipulation in sufficient detail for the non-specialist reader to understand them. However, in the five years since the last edition, molecular biologists have seen a dramatic increase in available techniques and applications. Every chapter in this fifth edition has been extensively revised or rewritten to take these developments into account - including a greatly expanded section on the polymerase chain reaction and its manifold applications; a new chapter examining the impact of recombinant DNA techniques on our understanding of human genetics; and extended coverage of gene transfer techniques. With entirely new illustrations in two colours throughout, this will remain the textbook of choice for undergraduates and postgraduates alike |
Notes |
Includes index |
Bibliography |
Includes bibliographical references (pages 412-464) and index |
Subject |
Recombinant DNA.
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Genetic engineering.
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DNA, Recombinant.
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Genetic Engineering.
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Author |
Primrose, S. B.
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LC no. |
94000932 |
ISBN |
0632037121 |
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