Title Quantitative PCR protocols / edited by Bernd Kochanowski and Udo Reischl

Published Totowa, N.J. : Humana Press, ©1999

Click on the following:
Springer eBooks

Copies

Description 1 online resource (x, 304 pages) : illustrations Series Methods in molecular medicine ; 26 Methods in molecular medicine ; 26. Contents General principles of quantitative PCR / L. Raeymaekers-Effects of collection, processing, and storage on RNA detection and quantification / M. Holodniy-Quantitative rt-PCR / P.D. Siebert-Kinetic quantitative PCR vs end-point quantitative PCR with inteRNAl standard / O. Lantz [and others]-Comparison of competitive PCR and positive control-based PCR / F. Mallet-End-point titration-PCR for quantitation of cytomegalovirus DNA / J.K. Kulski-Analysis of amplified DNA molecules by capillary electrophoresis and laser induced fluorescence / M.J. Fasco-Competitor calibration and analysis of competitive amplified PCR products by high-performance liquid chromatography (hplc) / T. Kohler-Quantifying amplicons with elisa / O. Lantz, E. Bonney and Y. Taoufik-Competitive PCR quantitation utilizing a microtiter plate based format for the detection of PCR products / B. Kochanowski and W. Jilg-Competitive and differential rt-PCR (cd-rt-PCR) for measurement of normalized gene expression using antisense competitors / E. de Kant-Amplified assay for specific dual-labeled DNA using the coagulation cascade (edna-elca) / G.J. Doellgast [and others]-Quantitative PCR with inteRNAl standardization and ola-elisa product analysis for the p53 tumor suppressor gene / M. Hahn and A. Pingoud-Quantitative analysis of human DNA sequences by PCR and solid-phase minisequencing / A. Suomalainen and A.C. Syvanen-High resolution PCR quantitation by amplisensor assay / C.N. Wang-Construction of polycompetitors for competitive PCR / D.B. Corry and R.M. Locksley-Tailed rt-PCR for the quantitation of chloramphenicol acetyl transferase (cat)mRNA / M.C. Knuchel and A.A. Ansari-A stochastic PCR approach for RNA quantification in multiple samples / A. Puntschart and M. Vogt-Quantitation of mRNA species by rt-PCR on total mRNA population with nonradioactive probes / S. Herblot, B. Rousseau and J. Bonnet Summary In Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. Quantitative PCR Protocols provides a collection of detailed methods that offers comprehensive and up-to-date information on how to control PCR to obtain reliable quantitation. With its step-by-step instructions researchers can really master the complexities of PCR and address otherwise inaccessible biological and diagnostic questions Analysis POLYMERASE CHAIN REACTION PCR POLYMERASES GENETIC MANIPULATION METHODS DNA RAN SYNTHESIS CHEMICAL REACTIONS MOLECULAR BIOLOGY Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Print version record In Springer Protocols Subject Polymerase chain reaction -- Laboratory manuals RNA. Methodology. DNA. Polymerase chain reaction. Nucleic acids. Genetics -- Technique. RNA Methods DNA Polymerase Chain Reaction Investigative Techniques Nucleic Acid Amplification Techniques Nucleic Acids Nucleic Acids, Nucleotides, and Nucleosides Analytical, Diagnostic and Therapeutic Techniques and Equipment Genetic Techniques Chemicals and Drugs methodology. SCIENCE -- Life Sciences -- Molecular Biology. Polymerase Chain Reaction. Biologie moléculaire. ADN = Acide désoxyribonucléique. RNA Nucleic acids Methodology Genetics -- Technique DNA Polymerase chain reaction ARN -- analyse. ADN -- analyse. Réaction de polymérisation en chaîne -- Méthodes. Biowissenschaften Aufsatzsammlung Polymerase-Kettenreaktion Quantitative Analyse Gen Polymerase kettingreactie. Wetenschappelijke technieken. ADN -- Analyse. Réaction en chaîne de la polymérase. Genre/Form Laboratory manuals Aufsatzsammlung. Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Kochanowski, Bernd. Reischl, Udo. ISBN 9781592592623 1592592627 0585239789 9780585239781 1280830514 9781280830518 9786610830510 6610830517

  Permalink