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Title Acute myeloid leukemia : methods and protocols / edited by Paolo Fortina, Eric Londin, Jason Y. Park, Larry J. Kricka
Published New York, N.Y. : Humana Press : Springer, ©2017


Description 1 online resource (xi, 278 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 1633
Methods in molecular biology (Clifton, N.J.) ; v. 1633
Contents Molecular malfeasance mediating myeloid malignancies : the genetics of acute myeloid leukemia / Rebecca L. King and Adam Bagg -- Cytochemical staining / Michele E. Paessler, Marybeth Helfrich, and Gerald B.W. Wertheim -- Immunohistochemistry in acute myeloid leukemia / Michael W. Cruise -- Immunophenotyping by multiparameter flow cytometry / Weina Chen and Hung S. Luu -- Single-cell mass cytometry of acute myeloid leukemia and leukemia stem/progenitor cells / Zhihong Zeng, Marina Konopleva, and Michael Andreeff -- Design and application of multiplex PCR seq for the detection of somatic mutations associated with myeloid malignancies / Naomi Park and George Vassiliou -- AMLprofiler : a diagnostic and prognostic microarray for acute myeloid leukemia / Marco Alessandrini, Sharon S. Kappala, and Michael S. Pepper -- Microsphere-based assessment of DNA methylation for AML prognosis / Gerald B.W. Wertheim, Marlise R. Luskin, Martin Carroll, and Stephen R. Master -- Genome-wide analysis of DNA methylation in hematopoietic cells : DNA methylation analysis by WGBS / Mira Jeong, Anna G. Guzman, and Margaret A. Goodell -- Detection and quantification of acute myeloid leukemia-associated fusion transcripts / Jonathan Schumacher, Philippe Szankasi, and Todd W. Kelley -- Targeted next-generation sequencing of acute leukemia / Eric Konnick, Christina M. Lockwood, and David Wu -- Bioinformatics data analysis of next-generation sequencing data from heterogeneous tumor samples / Sean R. Landman and Tae Hyun Hwang -- Zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation / Alvin C.H. Ma, Xiangguo Shi, Bai-Liang He, Yuhan Guo, and Anskar Y.H. Leung -- Measurement of oncometabolites d-2-hydroxyglutaric acid and l-2-hydroxyglutaric acid / Patricia M. Jones, Richard Boriack, Eduard A. Struys, and Dinesh Rakheja -- Nanofluidic allele-specific digital PCR method for quantifying IDH1 and IDH2 mutation burden in acute myeloid leukemia / Daniel H. Wiseman and Tim C.P. Somervaille -- Isolation of biologically active exosomes from plasma of patients with cancer / Chang-Sook Hong, Sonja Funk, and Theresa L. Whiteside -- Generating and expanding autologous chimeric antigen receptor T cells from patients with acute myeloid leukemia / Saad S. Kenderian, Carl H. June, and Saar Gill
Summary This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocols, viewed July 24, 2017)
Subject Acute myeloid leukemia -- Laboratory manuals
Acute myeloid leukemia.
Leukemia, Myeloid, Acute
Medical -- Oncology.
Acute myeloid leukemia
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Fortina, Paolo, editor
Londin, Eric, editor
Park, Jason Y., editor
Kricka, Larry J., editor
ISBN 9781493971428