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Title Adenovirus methods and protocols / edited by William S.M. Wold
Published Totowa, NJ : Humana Press, ©1999


Description 1 online resource (xiii, 352 pages) : illustrations
Series Methods in molecular medicine ; vol. 21
Methods in molecular medicine ; 21. 1543-1894
Contents Preparation and titration of CsCI-banded adenovirus stock / Ann E. Tollefson, Terry W. Hermiston, and William S.M. Wold -- Construction of mutations in adenovirus early region 3 (E3) transcription units / Terry W. Hermiston, Ann E. Tollefson, and William S.M. Wold -- Isolation, growth, and purification of defective adenovirus deletion mutants / Gary Ketner and Julie Boyer -- Manipulation of early region 4 / Julie Boyer and Gary Ketner -- Adenovirus DNA packaging: construction and analysis of viral mutants / Susanne I. Schmid and Patrick Hearing -- Methods for creating and anlyzing adenovirus vectors that express proteins that act on the viral genome / C.S.H. Young, Andrea L. Nicolás, Heng Lu, and Patricia L. Munz -- Construction of mouse adenovirus type 1 mutants / Angela N. Cauthen and Katherine R. Spindler -- Generation of adenovirus-specific cytotoxic T lymphocytes / Time E. Sparer and Linda R. Gooding -- Measurement of macrophage-mediated cytolysis of adenovirus-infected cells -- Penelope J. Duerksen-Hughes and Linda R. Gooding -- Measurement of tumor necrosis factor (TNF) lysis of adenovirus-infected cells / Joanna L. Shisler and Linda R. Gooding -- Role of natural killer (NK) cells in immunity to adenoviruses / John M. Routes -- Determination of the transforming activities of adenovirus oncogenes / T. Subramanian and G. Chinnadural -- Cell survival and apoptosis assays for E1B 19K and Bcl-2 family members / T. Subramanian and G. Chinnadural -- In vitro transcription: probing the molecular functions of adenovirus regulatory proteins / Paul M. Loewenstein, Chao-Zhong Song, and Maurice Green -- Cell microinjection: in vivo analysis of the functional domains of viral regulatory proteins / Maurice Green, Andrew Thorburn, and Paul M. Loewenstein -- Immunoprecipitation of E1A-containg protein complexes / Elizabeth Moran and Peter Yaciuk -- Preparaton of splicing-competent nuclear extracts from adenovirus-infected cells / Oliver M̈ühlemann and Göran Akusjärvi -- Adenovirus entry into cells: a quantitative fluorescence microscopy approach / Urs F. Greger, Michel Y. Nakano, and Maarit Suomalainen -- Simultaneous detection of RNA and proteins in adenovirus-infected cells by fluorescence in situ hybridization (FISH) and immunostaining / Eileen Bridge -- Characterization of the adenovirus fiber protein / Jeffrey A. Engler, Barbara Mulach, and Jeong Shin Hong -- Large-scale purification and crystallization of adenovirus hexon / John J. Rux, Donatella Pascolini, and Roger M. Burnett -- Adenovirus protease / Joseph M. Weber -- Methods for growth and purification of enteric adenovirus type 40 / Vivien Mautner -- Transfection complexes generated with adenovirus and polyethylenimine-condensed DNA / Matt Cotten, Mediyha Saltik, and Adam Baker -- Phylogenetic analysis of adenovirus sequences: proof of the necessity of establishing a third genus in the adenoviridae family / Balázs Harrach and Mária Benkö
Summary In Adenovirus Methods and Protocols, William S.M. Wold has organized a collection of readily reproducible methods for conducting research with adenoviruses, the premier and most widely used model in cell and molecular biology. The methods range from how to grow and titer adenoviruses and how to construct specific alterations in the adenovirus genome, to how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. In addition, there are methods to study transcription and splicing with in vitro systems and for the adenovirus-mediated transformation of cells to a malignant state. Each method is written by a prominent investigator well-versed in the technique and includes a brief background discussion, as well as tried and true step-by-step instructions. Adenovirus Methods and Protocols will be useful to both entry-level and senior scientists seeking to enter the adenovirus field, to researchers from other areas wishing to construct adenovirus vectors for their own research, and to adenovirologists wanting to enter new sectors of research. Its cutting-edge techniques are certain to make it today's reference of choice, one from which even expert researchers will learn many productive and time-saving techniques
Analysis adenoviridae
moleculaire biologie
molecular biology
genetische modellen
genetic models
laboratory methods
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. MiAaHDL
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
Subject Adenoviruses -- Laboratory manuals
Molecular biology -- Laboratory manuals
Molecular biology
Wetenschappelijke technieken.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Wold, William S. M.
ISBN 9781592596034