| Description |
1 online resource (xxx, 512 pages) : illustrations |
| Series |
Methods in enzymology, 0076-6879 ; v. 195 |
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Methods in enzymology ; v. 195. 0076-6879
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| Contents |
t Assay of adenylyl cyclase catalytic activity / r Roger A. Johnson ... [et al.] -- t Cellular responsiveness to hormones and neutrotransmitters: Conversion of [3H]Adenine to [3H]cAMP in cell monolayers, cell suspensions, and tissue slices / r Yoram Salomon, Roger A. Johnson and Jackie D. Corbin -- t Preparation of [alpha]-32P-Labeled nucleoside triphosphates, nicotinamide adenine dinucleotide, and cyclic nucleotides for use in determining adenylyl and guanylyl cyclases and cyclic nucleotide phosphodiesterase / r Timothy F. Walseth ... [et al.] -- t Synthesis of forskolin-agarose affinity matrices / r Thomas Pfeuffer, Roger A. Johnson and Jackie D. Corbin -- t High-affinity binding sites for [3H]forskolin / r Antonio Laurenza ... [et al.] -- t Purification and characterization of calmodulin-sensitive adenylyl cyclase from bovine brain / r Ping Wang ... [et al.] -- t Purification of adenylyl cyclase from heart and brain / r Elke Pfeuffer ... [et al.] -- t Calmodulin-mediated adenylyl cyclase from equine sperm / r William A. Toscano ... [et al.] -- t Isolation of polyclonal antibodies against bovine brain calmodulin-sensitive adenylyl cyclases / r Gary B. Rosenberg ... [et al.] -- t Characteristics and use of monoclonal antibodies to various forms of adenylyl cyclase / r Stefan Mollner ... [et al.] -- t Purification of soluble form of adenylyl cyclase from testes / r Theodor Braun, Roger A. Johnson and Jackie D. Corbin -- t Purification and assay of cell-invasive form of calmodulin-sensitive adenylyl cyclase from Bordetella pertussis / r H. Robert Masure ... [et al.] -- t Purification and characterization of adenylyl cyclase from Bacillus anthracis / r Stephen H. Leppla, Roger A. Johnson and Jackie D. Corbin -- t Preparation and application of GTP-agarose matrices / r Elke Pfeuffer ... [et al.] -- t Purification of G proteins / r Juan Codina ... [et al.] -- t Preparation of guanine nucleotide-free G proteins / r Kenneth M. Ferguson ... [et al.] -- t Purification of recombinant Gs[alpha] / r Michael P. Graziano ... [et al.] -- t Purification of recombinant G1[alpha] and Go[alpha] proteins from Escherichia coli / r Maurine E. Linder ... [et al.] -- t Synthetic peptide antisera with determined specificity for G protein [alpha] or [beta] subunits / r Susanne M. Mumby ... [et al.] -- t Quantitation and purification of ADP-ribosylation factor / r Richard A. Kahn, Roger A. Johnson and Jackie D. Corbin -- t Soluble guanine nucleotide-dependent ADP-ribosylation factors in activation of adenylyl cyclase by cholera toxin / r Joel Moss ... [et al.] -- t ADP-ribosylation of G proteins with pertussis toxin / r Gregory S. Kopf ... [et al.] -- t Cholera toxin-catalyzed [32P]ADP-ribosylation of proteins / r D. Michael Gill ... [et al.] -- t Photoaffinity labeling of GTP-binding proteins / r Rolf Thomas ... [et al.] |
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t Identification of receptor-activated G proteins with photoreactive GTP analog, [[alpha]-32P]GTP azidoanilide / r Stefan Offermanns ... [et al.] -- t Quantitative immunoblotting of G-protein subunits / r Donna J. Carty ... [et al.] -- t Assay of g-protein [beta][gamma]-subunit complex by catalytic support of ADP-ribosylation of Go[alpha] / r Patrick J. Casey ... [et al.] -- t Tryptophan fluorescence of G proteins: Analysis of guanine nucleotide binding and hydrolysis / r Tsutomu Higashijima ... [et al.] -- t Reconstitution of receptors and G proteins in phospholipid vesicles / r Richard A. Cerione ... [et al.] -- t Assay of guanylyl cyclase catalytic activity / r Steven E. Domino ... [et al.] -- t Preparative polyacrylamide gel electrophoresis apparatus for purification of guanylyl cyclase / r Alexander Mul̈sch ... [et al.] -- t Detergent interactions and solubilization techniques for membrane guanylyl cyclase / r Arnold A. White ... [et al.] -- t Purification of membrane form of guanylyl cyclase / r Chodavarapu S. Ramarao ... [et al.] -- t Purification of heme-containing soluble guanylyl cyclase / r Alexander Mul̈sch ... [et al.] -- t Preparation of soluble guanylyl cyclase from bovine lung by immunoaffinity chromatography / r Peter Humbert ... [et al.] -- t Immunoaffinity purification of soluble guanylyl cyclase / r Scott A. Waldman ... [et al.] -- t Copurification of atrial natriuretic peptide receptor and particulate guanylyl cyclase / r Scott A. Waldman ... [et al.] -- t Copurification of atrial natriuretic factor receptors and guanylyl cyclase from adrenal cortex / r Tadashi Inagami ... [et al.] -- t Molecular cloning of membrane forms of guanylyl cyclase / r Sujay Singh ... [et al.] -- t Radiation-inactivation analysis of multidomain proteins: The case of particulate guanylyl cyclase / r Michel Potier ... [et al.] -- t Identification of atrial natriuretic peptide receptors in cultured cells / r Dale C. Leitman ... [et al.] -- t Evaluating atrial natriuretic peptide-induced cGMP production by particulate guanylyl cyclase stimulation in Vitro and in Vivo / r Pavel Hamet ... [et al.] -- t Phosphorylation and dephosphorylation of sea urchin sperm cell guanylyl cyclase / r J. Kelley Bentley, Roger A. Johnson and Jackie D. Corbin -- t Calcium-regulated guanylyl cyclases from Paramecium and Tetrahymena / r Joachim E. Schultz ... [et al.] |
| Summary |
This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. Key Features * Adenylyl cyclase: determination of activity * Preparation of materials useful in purification of components of hormonally responsive cyclase systems * Purification guanine nucleotide-dependent regulatory proteins * Purification and characterization of g proteins: labeling and quantitating of G proteins * Reconstitution guanylyl cyclase: assay * Preparation of materials used for study of the cyclase * Purification * Regulation |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Description based on print version record |
| Subject |
Adenylate cyclase.
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G proteins.
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Adenylyl Cyclases -- isolation & purification.
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GTP-Binding Proteins -- isolation & purification.
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Guanylyl Imidodiphosphate -- isolation & purification.
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| Form |
Electronic book
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| Author |
Corbin, Jackie D.
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Johnson, Roger A
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| ISBN |
0121820963 (electronic bk.) |
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9780121820961 (electronic bk.) |
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