| Description |
1 online resource (xi, 247 pages) : illustrations |
| Series |
Methods in molecular biology, 1940-6029 ; 1089 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1089. 1064-3745
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| Contents |
Biophysical methods to monitor structural aspects of the adenovirus infectous cycle / Rosa Menéndez-Conejero [and others] -- Proteome analysis of adenovirus using mass spectrometry / Sara Bergström Lind, Konstantin A. Artemenko, and Ulf Pettersson -- Capsid modification strategies for detargeting adenoviral vectors / Alan L. Parker [and others] -- Use of dodecahedron "VLPs" as an alternative to the whole adenovirus / Pascal Fender -- Study of adenovirus and CAR axonal transport in primary neurons / Charleine Zussy and Sara Salinas -- The use of chromatin immunoprecipitation (hIP) to study the binding of viral proteins to the adenovirus genome in vivo / Yueting Zheng and Patrick Hearing -- DNA microarray to analyze adenovirus-host interactions / Stefania Piersanti, Enrico Tagliafico, and Isabella Saggio -- Determination of the transforming activities of adenovirus oncogenes / Thomas Speiseder, Michael Nevels, and Thomas Dobner -- Oncolytic adenovirus characterization : activity and immune responses / Raul Gil-Hoyos, Juan Miguel-Camacho, and Ramon Alemany -- The analysis of innate immune response to adenovirus using antibody arrays / Nelson C. Di Paolo and Dmitry M. Shayakhmetov -- Engineering adenovirus genome by bacterial artificil chromosome (BAC) technology / Zsolt Ruzsics, Frederic Lemnitzer, and Christian Thirion -- Construction, production, and purification of recombinant adenovirus vectors / Susana Miravet [and others] -- Sealable production of adenovirus vectors / Ana Carina Silva [and others] -- Canine adenovirus downstream processing protocol / Meritxell Puig [and others] -- Production of high-capacity adenovirus vectors / Florian Kreppel -- Production of chimeric adenovirus / Marta Miralles [and others] |
| Summary |
Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Adenoviruses -- Laboratory manuals
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Molecular biology -- Laboratory manuals
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DNA viruses.
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Viruses.
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Adenoviruses.
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DNA Viruses
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Vertebrate Viruses
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Viruses
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Organisms
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Adenoviridae
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Viruses
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DNA viruses
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Adenoviruses
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Molecular biology
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Chillón, Miguel, 1966- editor.
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Bosch, Assumpció, editor
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| ISBN |
9781627036795 |
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1627036792 |
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1627036784 |
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9781627036788 |
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